Polyhydroxyalkanoates (PHA) are biopolymer synthesized as storage granules by microbes in the cell’s cytoplasm under excess carbon and limited nitrogen conditions. This study aims to examine the production and optimization of PHA from Ceiba pentandra oil, a non-edible feedstock, by Cupriavidus necator. Response surface methodology was used to optimize the concentrations of carbon, nitrogen, and inoculum for the production of PHA. To improve the physical, mechanical, and thermal properties of PHA, it was blended with polylactic acid (PLA) which possesses high bio-compatibility and bio-absorbability. Furthermore, batch mode cultivation of PHA in a 3-L bioreactor was performed under the optimized conditions (Ceiba pentandra oil—17.13 g/L, urea—1.34 g/L, and inoculum—53.42 mL/L), and 8.51 g/L (0.47 g PHA/g of Ceiba pentandra oil) of PHA product was obtained. The PHA was characterized by FTIR and the presence of functional groups such as C=O, OH, and CH were observed. ¹H and ¹³C NMR spectroscopy revealed the existence of hydroxy valerate (HV) and hydroxybutyrate (HB), and maximal decomposition temperature was identified to be 217.7 °C from TGA analysis. Therefore, Ceiba pendantra oil appears to be an attractive alternate carbon source for PHA production.

Graphical abstract

聚羟基链烷酸酯（PHA）是生物聚合物，在过量碳和有限氮条件下，通过细胞质中的微生物合成为存储颗粒。这项研究旨在探讨PHA的从生产和优化吉贝油，非食用原料，由钩虫贪铜菌。响应面方法用于优化生产PHA的碳，氮和接种物的浓度。为了改善PHA的物理，机械和热性能，将其与具有高生物相容性和生物吸收性的聚乳酸（PLA）混合。此外，在优化的条件下，在3-L生物反应器中进行了PHA的分批培养（Ceiba pentandra得到油-17.13g / L，尿素-1.34g / L和接种物-53.42mL / L）和8.51g / L（0.47g PHA / g木棉五味子油）。通过FTIR对PHA进行表征，并观察到官能团（例如C = O，OH和CH）的存在。¹ H和¹³ C NMR光谱显示存在羟基戊酸（HV）和羟基丁酸（HB），并且通过TGA分析确定最高分解温度为217.7°C。因此，木棉侧柏油似乎是生产PHA的有吸引力的替代碳源。

图形概要