In this work, volatile fatty acids (VFAs) were used as a carbon source to assess the ability of bacteria present in waste activated sludge (WAS), as indigenous flora, to accumulate polyhydroxyalkanoates (PHA). Acetic acid and propionic acid were used both separately and in combination as feedstock, producing either homopolymer poly(3-hydroxybutyrate) (3PHB) and/or the co-polymer, poly(3-hydroxybutyrate- co -3-hydroxyvalerate) P(3HB-co-3HV). The overall potential to use waste activated sludge as biomass for production of valuable polymers was assessed, and a quality assessment of the as-produced polymers was run, with the extracted polymer being analyzed for properties such as thermal, microstructure and molecular weight. It was found that a blend of copolymers was typically produced, with thermal properties being similar to those reported elsewhere. The overall PHA cell content obtained was 0.29 gPHA gVSS −1 .

中文翻译：

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使用废弃活性污泥作为生物质在开放式混合培养中生产聚羟基链烷酸酯 (PHA)

在这项工作中，挥发性脂肪酸 (VFA) 被用作碳源，以评估存在于废弃活性污泥 (WAS) 中的细菌作为本地菌群积累聚羟基链烷酸酯 (PHA) 的能力。乙酸和丙酸单独或组合用作原料，生产均聚物聚（3-羟基丁酸酯）（3PHB）和/或共聚物，聚（3-羟基丁酸酯-co-3-羟基戊酸酯）P(3HB) -co-3HV）。评估了使用废弃活性污泥作为生物质生产有价值聚合物的总体潜力，并对所生产的聚合物进行了质量评估，并对提取的聚合物进行了热、微观结构和分子量等特性分析。发现通常会产生共聚物的共混物，热性能与其他地方报道的相似。获得的总PHA细胞含量为0.29 gPHA gVSS -1 。