As a part of studying the effect of deoxygenation, eutrophication and acidification on bacterial diversity, strain HWN-4T was isolated from tube well water and characterized. The draft genome sequencing of strain HWN-4T revealed a genome size of 5,774,764 bp and the annotation indicated 5102 coding sequences including 66 RNA genes. Strain HWN-4T is Gram negative, rod-shaped, motile in the log phase, catalase and oxidase positive, and the major fatty acids and respiratory quinone present are C10:0 3-OH, C14:0 3OH/C16:1 iso I, C16:1 ω7c/C16:1 ω6c, C16:0 and C17:0 cyclo and ubiquinone-8, respectively. The phylogenetic analyses, based on 16S rRNA gene sequence, indicated that strain HWN-4T is a member of the genus Mitsuaria. The average nucleotide identity (ANI) and genome-to-genome similarity between strain HWN-4T and all other species/strains of the genus Mitsuaria are less than (%) 95.0 and 70.0, respectively. This confirms the status of strain HWN-4T as a novel species. The species status is further confirmed by phenotypic differences exhibited by strain HWN-4T with other members of the same genus. Based on the collective differences exhibited by strain HWN-4T with other members of the genus Mitsuaria, the name Mitsuaria chitinivorans sp. nov. is proposed. Further, the diagnostic signature nucleotides were identified in the 16S rRNA gene sequences of members of the genera Mitsuaria, Pelomonas and Roseateles, that distinctly differentiate them and support an emendation of the genera. Besides, phylogenetic and structural characterization of chitinases from members of the genus Mitsuaria was performed. The type strain of Mitsuaria chitinivorans sp. nov. is HWN-4T = LMG 28685T = KTCC 42483T.

中文翻译：

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三叶螟 十一月 生物修复的潜在候选者：修正了对 Mitsuaria、Roseateles 和 Pelomonas 属的描述

作为研究脱氧、富营养化和酸化对细菌多样性影响的一部分，从管井水中分离出菌株 HWN-4T 并对其进行了表征。菌株 HWN-4T 的基因组测序草图显示基因组大小为 5,774,764 bp，注释显示 5102 个编码序列，包括 66 个 RNA 基因。菌株 HWN-4T 为革兰氏阴性、杆状、对数期运动、过氧化氢酶和氧化酶阳性，存在的主要脂肪酸和呼吸醌为 C10:0 3-OH、C14:0 3OH/C16:1 iso I , C16:1 ω7c/C16:1 ω6c, C16:0 和 C17:0 环和泛醌-8，分别。基于 16S rRNA 基因序列的系统发育分析表明，菌株 HWN-4T 是 Mitsuaria 属的成员。菌株 HWN-4T 与所有其他种类/菌株 Mitsuaria 之间的平均核苷酸同一性 (ANI) 和基因组相似性分别小于 (%) 95.0 和 70.0。这证实了菌株 HWN-4T 作为新物种的地位。菌株 HWN-4T 与同一属的其他成员表现出的表型差异进一步证实了物种状态。基于菌株 HWN-4T 与 Mitsuaria 属其他成员表现出的集体差异，名称 Mitsuaria chitinivorans sp。十一月 被提议。此外，在Mitsuaria、Pelomonas 和Roseateles 属成员的16S rRNA 基因序列中鉴定出诊断特征核苷酸，可以明显地区分它们并支持该属的修正。除了，对来自 Mitsuaria 属成员的几丁质酶进行了系统发育和结构表征。Mitsuaria chitinivorans sp. 的典型菌株。十一月 是 HWN-4T = LMG 28685T = KTCC 42483T。