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Assessment of changes in the content of sulforaphane and expression levels of CYP79F1 and myrosinase genes and proteomic profile of Lepidium draba plant under water-deficit stress induced by polyethylene glycol
Acta Physiologiae Plantarum ( IF 2.6 ) Pub Date : 2020-05-24 , DOI: 10.1007/s11738-020-03085-1
Kiarash Jamshidi Goharrizi , Foad Fatehi , Maryam Nazari , Fatemeh Salehi , Mahmood Maleki

Lepidium draba weed, which has a very valuable secondary metabolite named sulforaphane, is a precious medicinal plant that very few studies have been done on it. In this study, 5-day-old Lepidium draba plants were grown for 9 days in differentially levels of polyethylene glycol and the amount of total Phenols, total Flavonoids, total Anthocyanins, sulforaphane, chlorophyll and Carotenoids as well as cytochorome P450 79F1 and myrosinase genes expression levels, which are two main genes in the biosynthesis pathway of sulforaphane, were investigated in14-day-old sprouts of these plants. Our results showed that with increasing doses of polyethylene glycol, the contents of total Phenols, total Flavonoids, total Anthocyanins and sulforaphane increased, while chlorophyll and Carotenoids contents decreased. In addition, cytochorome P450 79F1 and myrosinase genes expression levels increased with enhancement of polyethylene glycol levels. A direct relationship was observed between sulforaphane content and cytochorome P450 79F1 and myrosinase genes expression together and also with enhancement of polyethylene glycol concentrations. To determine the protein changes in the Lepidium draba plant under water-deficit stress, a proteomics study was carried out on total 14-day-old sprouts of this plant by two-dimensional electrophoresis (2-DE) based on proteomics method under non stress (polyethylene glycol 0%), and medium stress (polyethylene glycol 6%) conditions. We have for the first time found that glutamate 5-kinase, 3′-5′ exonuclease and Sigma-54-dependent Fis family transcriptional regulator, were changed remarkably in plant in response to abiotic stress.



中文翻译:

聚乙二醇引起的水分亏缺胁迫下dr草植物萝卜硫素含量,CYP79F1和黑芥子酶基因表达水平及蛋白质组学特征的变化

淫羊dr杂草具有非常有价值的次生代谢产物萝卜硫烷,是一种珍贵的药用植物,很少对其进行研究。在这项研究中,5天大的Lepidium draba植物在不同水平的聚乙二醇和总酚,总黄酮,总花色苷,萝卜硫烷,叶绿素和类胡萝卜素以及细胞色素P450 79F1和黑芥子酶基因表达水平的差异水平下生长9天,这是生物合成中的两个主要基因在这些植物的14天大的芽中研究了萝卜硫烷的途径。我们的结果表明,随着聚乙二醇剂量的增加,总酚,总黄酮,总花青素和萝卜硫素的含量增加,而叶绿素和类胡萝卜素的含量下降。此外,细胞膜P450 79F1和黑芥子酶基因表达水平随聚乙二醇水平的提高而增加。观察到萝卜硫烷含量与细胞吞噬激素P450 79F1和黑芥子酶基因一起表达以及与聚乙二醇浓度增加之间存在直接关系。要确定蛋白质的变化干旱胁迫下的dr鳞草(Lepidium draba)植物,通过基于蛋白质组学方法的二维电泳(2-DE),在无胁迫(聚乙二醇0%)的条件下对该植物的14天大的新芽进行了蛋白质组学研究,和中等压力(聚乙二醇6%)条件。我们首次发现谷氨酸5-激酶,3'-5'核酸外切酶和依赖于Sigma-54的Fis家族转录调节剂在植物中响应非生物胁迫而发生了显着变化。

更新日期:2020-05-24
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