Class A saponins are responsible for the taste of soybean products, and the rapid identification of class A saponins from soybean food is essential for both food safety and cultivar screening. In this study, we propose a colorimetric assay based on the coupling of gap ligase chain reaction (Gap-LCR) with DNAzyme to detect the target GmSg-1 genes of class A soybean saponins with the naked eye, without the involvement of expensive instruments. The limits of detection (LODs) for the GmSg-1^a and GmSg-1^b genes were determined to be 0.1618 and 0.1625 μM, respectively, with a linear range of 0.2–1.2 μM. The DNAzyme-based Gap LCR assay was successfully employed to identify the target genes from different soybean cultivars, providing a simple means for monitoring the quality of soybean products.

中文翻译：

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通过将DNA酶与空位连接酶链反应偶联，比色检测A类大豆皂苷。

A类皂苷负责大豆产品的味道，从大豆食品中快速识别A类皂苷对于食品安全和品种筛选至关重要。在这项研究中，我们提出了基于间隙连接酶链反应（Gap-LCR）与DNAzyme偶联的比色测定法，用肉眼即可检测A类大豆皂苷的目标GmSg-1基因，而无需使用昂贵的仪器。GmSg-1 ^a和GmSg-1 ^b的检出限（LOD）基因被确定为分别为0.1618和0.1625μM，线性范围为0.2–1.2μM。基于DNA酶的Gap LCR分析成功地用于鉴定来自不同大豆品种的靶基因，为监测大豆产品的质量提供了一种简单的方法。