This paper reports a dual electrochemical biosensor involving carboxylated- or neutravidin-functionalized magnetic microbeads and dual screen-printed carbon electrodes for the simultaneous determination of rheumatoid factor (RF) and anti-cyclic citrullinated peptide (CCPA) autoantibodies used as biomarkers for the detection of rheumatoid arthritis autoimmune disease. Sandwich-type biosensors involving Fc fragments of IgG Fc(IgG) and biotinylated cyclic cytrullinated peptide (CCP-biotin) to form CCP-biotin-Neutr-MBs for the specific immobilization of RF and CCPA, respectively, as well as conjugation with HRP-IgM and HRP-IgG for RF and CCPA, respectively, were prepared. Amperometric detection was performed at −0.20 V vs. Ag pseudo-reference electrode using the H₂O₂/hydroquinone (HQ) system upon capturing the bioconjugates onto the corresponding working electrode (WE1 or WE2) of SPCdEs. The dual biosensor exhibits high sensitivity for RF and CCPA with LOD values of 0.8 and 2.5 IU mL⁻¹, respectively. The simultaneous determination can be completed in about two hours using a simple protocol and a sample volume (25 μL) four times smaller than that required by the ELISA method. The dual electrochemical biosensor was used for the determination of both target biomarkers in human serum.

中文翻译：

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电化学生物传感器，可同时测定人血清中的类风湿因子和抗环瓜氨酸肽抗体。

本文报道了一种双电化学生物传感器，包括羧基化或中性亲和素官能化的磁性微珠和双丝网印刷的碳电极，用于同时测定类风湿因子（RF）和抗环瓜氨酸肽（CCPA）自身抗体作为生物标志物，以检测类风湿关节炎自身免疫性疾病。包含IgG Fc（IgG）Fc片段和生物素化环化瓜氨酸肽（CCP-生物素）的夹心型生物传感器，形成CCP-生物素-Neutr-MB，分别用于RF和CCPA的特异性固定，以及与HRP-分别制备了用于RF和CCPA的IgM和HRP-IgG。使用H ₂ O ₂在-0.20 V vs. Ag伪参比电极上进行安培检测在将生物结合物捕获到SPCdE的相应工作电极（WE1或WE2）上时，使用对苯二酚/对苯二酚（HQ）系统。双重生物传感器对RF和CCPA表现出高灵敏度，LOD值分别为0.8和2.5 IU mL ^-1。可以使用简单的方案在大约两个小时内完成同时测定，样品体积（25μL）比ELISA方法所需的体积小四倍。双电化学生物传感器用于测定人血清中的两种靶标生物标志物。