Abstract

In this study, an optimal method used to extract Annona squamosa pericarp oil (ASPO) was established according to the response surface model. The yield of ASPO was 1.45%. 8 fatty acids were identified from ASPO by GC-MS. Among them, (9Z)-9-Octadecenoic acid was abundant and accounted for 49.65%. The anti-hepatoma activities of ASPO were investigated against SMMC-7721 cell line in vitro and H₂₂ cell line in vivo. Proteins associated with apoptosis in tumour tissue were quantified by western blot assay. The result revealed that ASPO had significant anti-hepatoma activities with IC₅₀ value of 15.96 μg/mL in vitro and tumour inhibition rate of 54.14% at 50 mg/kg dose in vivo. Protein analysis showed that ASPO activated apoptosis by down-regulating Bcl-2, up-regulating Bax, cleaving caspase 9, cleaving caspase 8 and cleaving caspase 3 proteins. The possible mechanisms of apoptosis induced by ASPO were related to Fas/FasL/caspase-8/caspase-3 and Bcl-2/bax/caspase-9/caspase-3 pathways.

摘要

本研究根据响应面模型建立了番荔枝果皮油（ASPO）的最佳提取方法。ASPO的产率为1.45%。通过GC-MS从ASPO中鉴定出8种脂肪酸。其中( 9Z )-9-十八碳烯酸含量丰富，占49.65%。在体外对 SMMC-7721 细胞系和体内H ₂₂细胞系研究了 ASPO 的抗肝癌活性。通过蛋白质印迹分析定量与肿瘤组织中的细胞凋亡相关的蛋白质。结果表明，ASPO具有显着的抗肝癌活性，体外IC ₅₀值为15.96 μg/mL50 mg/kg体内抑瘤率为54.14% 。蛋白质分析表明，ASPO 通过下调 Bcl-2、上调 Bax、裂解 caspase 9、裂解 caspase 8 和裂解 caspase 3 蛋白激活细胞凋亡。ASPO诱导细胞凋亡的可能机制与Fas/FasL/caspase-8/caspase-3和Bcl-2/bax/caspase-9/caspase-3通路有关。