Abstract The objective of this study was to evaluate the physico-chemical and antimicrobial properties of a dual polymerization experimental endodontic sealer (E) and experimental sealers containing dibutyltin methacrylate (Sn2+) (ETs) or calcium methacrylate (Ca2+) (ECs). The pH and ion release levels of the sealers were measured. The dimensional stability was evaluated in accordance with ISO 6876. Biofilm growth inhibition was evaluated using confocal laser scanning microscopy (CLSM). Biofilm viability analysis was performed using the SYTO 9 technique. The shelf life was evaluated through the degree of conversion and film thickness tests after the sealers had been stored for different periods of time. For statistical analysis, ANOVA and Tukey’s post hoc test were used, with a significance level of 5%. ETs revealed better anti-biofilm potential after 15 days than that of the controls. The degree of conversion was reduced after the shelf-life period. The addition of calcium and dibutyltin methacrylate improved the anti-biofilm properties of the experimental endodontic sealer without impairing their physico-chemical properties.

中文翻译：

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含有金属甲基丙烯酸酯的实验性牙髓封闭剂的物理化学和抗菌特性以及保质期

摘要 本研究的目的是评估双重聚合实验性牙髓封闭剂 (E) 和含有甲基丙烯酸二丁基锡 (Sn2+) (ETs) 或甲基丙烯酸钙 (Ca2+) (ECs) 的实验性封闭剂的理化和抗菌性能。测量了封闭剂的 pH 值和离子释放水平。根据 ISO 6876 评估尺寸稳定性。使用共聚焦激光扫描显微镜 (CLSM) 评估生物膜生长抑制。使用 SYTO 9 技术进行生物膜活力分析。保质期是通过密封剂储存不同时间后的转化程度和膜厚测试来评估的。对于统计分析，使用方差分析和 Tukey 事后检验，显着性水平为 5%。ET 在 15 天后显示出比对照更好的抗生物膜潜力。保质期后转化程度降低。添加钙和甲基丙烯酸二丁基锡提高了实验性牙髓封闭剂的抗生物膜特性，而不会损害其理化特性。