The Pantoea agglomerans 8488 lipopolysaccharide (LPS) was isolated, purified and characterized by monosaccharide and fatty acid analysis. The O-polysaccharide and lipid A components of the LPS were separated by mild acid degradation. Lipid A was studied by electrospray ionization mass spectrometry (ESI-MS) and found to consist of hexa-, penta-, tetra- and tri-acylated species. Two-dimensional nuclear magnetic resonance (NMR) spectroscopy revealed the following structure of the O-polysaccharide repeating unit →3)-α-L-Rhap-(1→6)-α-D-Manp-(1→3)-α-L-Fucp-(1→3)-β-D-GlcNAcp-(1→. The LPS showed a low level of toxicity, was not pyrogenic, and reduced the adhesiveness index of microorganisms to 2.12, which was twofold less than the control. LPS modified by complex compounds of germanium (IV) and tin (IV) were obtained. It was found that six LPS samples modified by Sn compounds and two LPS samples modified by Ge compounds lost their toxic activity when administered to mice in a dose of LD₅₀ (105 µg/mice or 5 mg/kg). However, none of the modified LPS samples changed their serological activity in an Ouchterlony double immunodiffusion test in agar.

中文翻译：

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Pantoea Agglomerans 8488 的 O-多糖和脂质 A 的结构。

通过单糖和脂肪酸分析分离、纯化并表征了成团泛菌8488脂多糖 (LPS)。LPS 的 O-多糖和脂质 A 组分通过温和的酸降解分离。通过电喷雾电离质谱 (ESI-MS) 对脂质 A 进行了研究，发现其由六酰化、五酰化、四酰化和三酰化物质组成。二维核磁共振 (NMR) 光谱揭示了 O-多糖重复单元 →3)-α-L-Rha p- (1→6)-α-D-Man p- (1→3)的以下结构-α-L-Fuc p -(1→3)-β-D-GlcNAc p -(1→。LPS毒性低，不致热原，可将微生物的粘附指数降低至2.12，获得了经锗(IV)和锡(IV)复合化合物修饰的LPS，发现6个Sn化合物修饰的LPS样品和2个Ge化合物修饰的LPS样品在给药后失去了毒性活性。以 LD ₅₀（105 µg/小鼠或 5 mg/kg）剂量对小鼠进行实验。然而，在 Ouchterlony 琼脂双重免疫扩散试验中，修饰的 LPS 样品均未改变其血清学活性。