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Prenatal histomorphological development of the reticulum in fallow deer (Dama dama).
Animal Science Journal ( IF 2 ) Pub Date : 2020-05-22 , DOI: 10.1111/asj.13319
Eloy Redondo 1 , Ángela García 1 , Cristina Ortega 2 , Fernando J Peña 2 , Antonio Gázquez 1 , Javier Masot 1
Affiliation  

The histomorphological changes occurring in the Dama dama reticulum during prenatal development have been investigated. Twenty-five Dama dama embryos were used, from the first stages of prenatal life until birth. Differentiation of the reticulum was observed at 23% gestation. By 25% gestation the reticular wall comprised three layers: an internal epithelial layer, a middle layer of pluripotential blastemic tissue and an external layer or serosa. Primary reticular crests were visible at 38% gestation. Secondary reticular crests were observed at 61% gestation. Neuroendocrine cells were detected by synaptophysin (SYP) at 35% gestation, in the lamina propria-submucosa, tunica muscularis, and serosa. Epithelial Cytokeratin-18 (CK-18) cells were observed at 35% gestation extended throughout the epithelial layers. The glial cells (vimentin -VIM- and glial fibrillary acidic protein-GFAP-markers) were discerned at 25% and 43% gestation, respectively, in myenteric and submucosal plexuses, lamina propria, muscularis mucosae, tunica muscularis, and perivascular connective tissue. The neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP) markers were immunodetected at 75% and 80 gestation, respectively, in the lamina propria-submucosa, muscularis mucosae, tunica muscularis, serosa, and myenteric plexuses. The prenatal development of the fallow deer reticular mucosa evidenced a considerable precocity similar to that previously reported in goat and red deer.

中文翻译:

小鹿 (Dama dama) 网状结构的产前组织形态学发育。

已经研究了产前发育过程中 Dama dama 网状结构发生的组织形态学变化。从产前生命的第一阶段到出生,使用了 25 个 Dama dama 胚胎。在 23% 妊娠时观察到网状结构的分化。到 25% 妊娠时,网状壁由三层组成:内部上皮层、多能胚细胞组织的中间层和外层或浆膜层。妊娠 38% 时可见初级网状嵴。在 61% 妊娠时观察到次级网状嵴。在妊娠 35% 时,突触素 (SYP) 在粘膜固有层、粘膜下层、肌层和浆膜中检测到神经内分泌细胞。在 35% 的妊娠期观察到上皮细胞角蛋白 18 (CK-18) 细胞扩展到整个上皮层。神经胶质细胞(波形蛋白-VIM-和神经胶质纤维酸性蛋白-GFAP-标志物)分别在妊娠 25% 和 43% 时在肌间神经丛和粘膜下神经丛、固有层、粘膜肌层、肌层和血管周围结缔组织中被辨别出来。神经肽 Y (NPY) 和血管活性肠多肽 (VIP) 标记物在 75% 和 80 妊娠时分别在粘膜固有层粘膜下层、粘膜肌层、肌层、浆膜和肌间神经丛中被免疫检测到。小鹿网状粘膜的产前发育证明了与先前在山羊和马鹿中报道的相似的相当早熟。和血管周围结缔组织。神经肽 Y (NPY) 和血管活性肠多肽 (VIP) 标记物在 75% 和 80 妊娠时分别在粘膜固有层粘膜下层、粘膜肌层、肌层、浆膜和肌间神经丛中被免疫检测到。小鹿网状粘膜的产前发育证明了与先前在山羊和马鹿中报道的相似的相当早熟。和血管周围结缔组织。神经肽 Y (NPY) 和血管活性肠多肽 (VIP) 标记物在 75% 和 80 妊娠时分别在粘膜固有层粘膜下层、粘膜肌层、肌层、浆膜和肌间神经丛中被免疫检测到。小鹿网状粘膜的产前发育证明了与先前在山羊和马鹿中报道的相似的相当早熟。
更新日期:2020-05-22
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