Environmental DNA (eDNA) analysis is a technique increasingly used for biomonitoring and research of fish populations and communities by environmental resource managers and academic researchers. Although there is much interest in expanding the use of eDNA as a survey technique for managers, there remains skepticism regarding both its utility (given that information is often limited to presence/absence of a species) and feasibility (given the need for proper lab facilities for sample processing). However, under the right circumstances, eDNA analysis is cost-effective compared to many traditional aquatic survey methods and does not cause disturbance to habitat or harm to the animals being surveyed. Here we present a case study in which eDNA analysis was successfully used to document the presence of a rare fish species in a waterway earmarked for restoration. We discuss the conditions that allowed this study to happen quickly and smoothly and speculate on how the goals of researchers and managers can be integrated for efficient and informative use of this tool. This article is protected by copyright. All rights reserved.

中文翻译：

---

寻找难以看到的地方：一个案例研究记录了加利福尼亚泻湖中罕见的 Eucyclogobius newberryi

环境 DNA (eDNA) 分析是一种越来越多地被环境资源管理者和学术研究人员用于鱼类种群和群落生物监测和研究的技术。尽管人们对扩大使用 eDNA 作为管理人员的调查技术很感兴趣，但对其效用（鉴于信息通常仅限于物种的存在/不存在）和可行性（考虑到需要适当的实验室设施）仍然持怀疑态度用于样品处理）。然而，在适当的情况下，与许多传统的水生调查方法相比，eDNA 分析具有成本效益，并且不会对栖息地造成干扰或对被调查动物造成伤害。在这里，我们展示了一个案例研究，其中 eDNA 分析成功地用于记录指定用于修复的水道中存在稀有鱼类。我们讨论了使这项研究能够快速顺利进行的条件，并推测如何整合研究人员和管理人员的目标，以有效地和信息丰富地使用该工具。本文受版权保护。版权所有。