Previous studies demonstrated that the pathophysiological changes after temporal lobe epilepsy (TLE) such as oxidative stress, inflammatory reaction contribute to cognitive defect and neuronal damage. The present study was conducted to evaluate the anticonvulsant effect of wogonin ameliorates kainate-induced TLE, and to investigate the mechanism underlying these effects. Rats were divided into control, wogonin, kainate, and wogonin-pretreated kainate groups. The rat model of TLE was induced by unilateral intrahippocampal injection of 0.4 ug/ul of kainate. The results showed that the cognitive function in TLE rats was significantly impaired, and wogonin treatment improved cognitive function in the Morris water maze (MWM). H & E staining and TUNEL staining showed obvious damage in the hippocampus of TLE rats, and wogonin alleviated the damage. To evaluate the oxidative stress, the expression of MDA and GSH in plasma were detected. Nrf-2 and HO-1 mRNA expression in the hippocampus were detected. The levels of MDA in plasma increased in TLE rats, and the levels of GSH in plasma and Nrf-2, HO-1 in the brain decreased. Treatment with wogonin alleviated these changes. We also detected the mRNA expression of inflammatory mediators like IL-1β, TNF-α, and NF kB in the brain. The inflammatory reaction was significantly activated in the brain of TLE rats, and wogonin alleviated neuroinflammation. We detected the mRNA expression of Bcl-2, Bax, caspase-3, in the hippocampus. The levels of Bcl-2 decreased in TLE rats, Bax and caspase-3 increased, while wogonin alleviated these changes. The present study indicated that wogonin exerted a noticeable neuroprotective effect in kainate-induced TLE rats.

先前的研究表明颞叶癫痫（TLE）后的病理生理变化，例如氧化应激，炎症反应，会导致认知缺陷和神经元损伤。本研究旨在评估wogonin改善海藻酸盐诱导的TLE的抗惊厥作用，并研究这些作用的潜在机制。将大鼠分为对照组，沃戈宁，海藻酸盐和沃戈宁预处理的海藻酸盐组。通过单侧海马内注射0.4 ug / ul的海藻酸盐来诱导TLE大鼠模型。结果表明，TLE大鼠的认知功能显着受损，而伍格宁治疗改善了莫里斯水迷宫（MWM）的认知功能。H＆E染色和TUNEL染色显示TLE大鼠海马明显受损，而wogonin减轻了损害。为了评估氧化应激，检测血浆中MDA和GSH的表达。检测海马中Nrf-2和HO-1 mRNA的表达。TLE大鼠血浆中MDA含量升高，血浆GSH含量，脑Nrf-2，HO-1含量下降。wogonin的治疗减轻了这些变化。我们还检测了大脑中IL-1β，TNF-α和NF kB等炎症介质的mRNA表达。TLE大鼠的大脑中炎症反应被显着激活，而沃戈宁减轻了神经炎症。我们在海马中检测到Bcl-2，Bax，caspase-3的mRNA表达。TLE大鼠中Bcl-2的水平降低，Bax和caspase-3的水平升高，而伍格宁减轻了这些变化。本研究表明，wogonin在海藻酸盐诱导的TLE大鼠中发挥了明显的神经保护作用。