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Systematic analysis of SmWD40s, and responding of SmWD40-170 to drought stress by regulation of ABA- and H2O2-induced stomal movement in Salvia miltiorrhiza bunge.
Plant Physiology and Biochemistry ( IF 6.5 ) Pub Date : 2020-05-22 , DOI: 10.1016/j.plaphy.2020.05.017
Yuan-Chu Liu 1 , Wen Ma 1 , Jun-Feng Niu 1 , Bin Li 1 , Wen Zhou 1 , Shuai Liu 1 , Ya-Ping Yan 1 , Ji Ma 1 , Zhe-Zhi Wang 1
Affiliation  

WD40 proteins play crucial roles in response to abiotic stress. By screening the genome sequences of Salvia miltiorrhiza Bunge, 225 SmWD40 genes were identified and divided into 9 subfamilies (I-IX). Physiological, biochemical, gene structure, conserved protein motif and GO annotation analyses were performed on SmWD40 family members. The SmWD40-170 was found in 110 SmWD40 genes that contain drought response elements, SmWD40-170 was one of these genes whose response in terms of expression under drought was significant. The expression of SmWD40-170 was also up-regulated by ABA and H2O2. Through observed the stomatal phenotype of SmWD40-170 transgenic lines, the stomatal closure was abolished under dehydration, ABA and H2O2 treatment in SmWD40-170 knockdown lines. Abscisic acid (ABA), as the key phytohormone, elevates reactive oxygen species (ROS) levels under drought stress. The ABA-ROS interaction mediated the generation of H2O2 and the activation of anion channel in guard cells. The osmolality alteration of guard cells further accelerated the stomatal closure. As a second messenger, nitric oxide (NO) regulated ABA signaling, the NO stimulated protein kinase activity inhibited the K+ influx which result in stomatal closure. These NO-relevant events were essential for ABA-induced stomatal closure. The reduction of NO production was also observed in the guard cells of SmWD40-170 knockdown lines. The abolished of stomatal closure attributed to the SmWD40-170 deficiency induced the reduction of NO content. In general, the SmWD40-170 is a critical drought response gene in SmWD40 gene family and regulates ABA- and H2O2-induced stomatal movement by affecting the synthesis of NO.



中文翻译:

系统分析SmWD40s,并通过调节丹参中ABA和H2O2诱导的气孔运动来调节SmWD40-170对干旱胁迫的反应。

WD40蛋白在应对非生物胁迫中起关键作用。通过筛选丹参丹参的基因组序列,鉴定出225个SmWD40基因并将其分为9个亚家族(I-IX)。对SmWD40家族成员进行了生理,生化,基因结构,保守的蛋白质基序和GO注释分析。该SmWD40-170在110倍发现SmWD40含有干旱应答元件的基因,SmWD40-170是这些基因,其响应在干旱条件下表达方面是显著之一。SmWD40-170的表达也被ABA和H 2 O 2上调。通过观察气孔表型SmWD40-170转基因品系,在脱水,ABA和H 2 O 2处理的SmWD40-170组合品系中,气孔关闭被取消。脱落酸(ABA)作为主要植物激素,可提高干旱胁迫下的活性氧(ROS)水平。ABA-ROS相互作用介导了H 2 O 2的生成和保卫细胞中阴离子通道的活化。保卫细胞的渗透压改变进一步加速了气孔关闭。作为第二信使,一氧化氮(NO)调节ABA信号传导,NO刺激的蛋白激酶活性抑制了K +大量涌入导致气孔关闭。这些与NO相关的事件对于ABA诱导的气孔关闭至关重要。在SmWD40-170敲低系的保卫细胞中也观察到NO生成的减少。SmWD40-170缺乏导致的气孔关闭被取消,导致NO含量降低。通常,SmWD40-170SmWD40基因家族中的关键干旱反应基因,通过影响NO的合成来调节ABA和H 2 O 2诱导的气孔运动。

更新日期:2020-05-22
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