In the present study bacterial strain (VITURAJ10), isolated from goat milk was characterised for its probiotic potential. The various probiotic traits included tolerance to acidic pH (up to pH 3), bile salts (0.3%) and transit gut environment (simulated with digestive juices such as pepsin, Oxgall and pancreatin). The isolate could withstand high NaCl concentrations in the growth medium, showed inability to produce hemolysin and did not hydrolyse mucin. VITURAJ10 was capable of forming biofilm and produced exopolysachharide. The bioactive metabolites produced by the isolate were extracted and they showed growth suppressing activity towards pathogenic strains such as Escherichia coli, Salmonella enterica and Staphylococcus aureus. The crude extract was fractionated with solid phase extraction (SPE) chromatography and the fractions 10 and 12 were found to be effective against the bacterial pathogens. The fractions were further gauged for cytotoxic activity against MCF-7 cell line by MTT assay. The biologically significant compounds identified through GC-MS and FT-IR analysis in the fractions were, Actinomycin D, Pyrrolo [1,2α] Pyrazine-1,4-Dione, Hexahydro-3-(2-Methylpropyl)- (PPDHMP) and Didemnin B. The phylogenetic taxonomy of the isolate revealed the isolate to be the closest neighbour of Staphylococcus xylosus VITURAJ10 (GenBank accession no. KX770743.1) as per the16S rRNA gene sequencing and subsequent phylogenetic tree analysis.

中文翻译：

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葡萄球菌VITURAJ10：产吡咯并[1,2α]吡嗪-1,4-二酮，六氢-3-（2-甲基丙基）（PPDHMP）的潜在益生菌菌株，具有抗菌和抗癌活性。

在本研究中，从山羊奶中分离出的细菌菌株（VITURAJ10）具有潜在的益生菌特性。各种益生菌性状包括对酸性pH值（高达pH 3）的耐受性，胆汁盐（0.3％）和肠道环境（用消化液，如胃蛋白酶，奥克斯加尔和胰酶模拟）。该分离株可以承受生长培养基中的高NaCl浓度，不能产生溶血素，并且不水解粘蛋白。VITURAJ10能够形成生物膜并产生胞外多糖。提取分离物产生的生物活性代谢物，它们对诸如大肠杆菌，肠炎沙门氏菌和金黄色葡萄球菌的病原菌株显示出抑制生长的活性。用固相萃取（SPE）色谱分离粗提物，发现馏分10和12对细菌病原体有效。通过MTT测定进一步评估级分对MCF-7细胞系的细胞毒活性。通过GC-MS和FT-IR分析鉴定出的馏分中具有生物学意义的化合物为放线菌素D，吡咯罗[1,2α]吡嗪-1,4-二酮，六氢-3-（2-甲基丙基）-（PPDHMP）和Didemnin B.根据16S rRNA基因测序和随后的系统进化树分析，该分离株的系统进化分类学表明该分离株是葡萄球菌VITURAJ10（GenBank登录号KX770743.1）的最近邻居。通过MTT测定进一步评估级分对MCF-7细胞系的细胞毒活性。通过GC-MS和FT-IR分析鉴定出的馏分中具有生物学意义的化合物为放线菌素D，吡咯罗[1,2α]吡嗪-1,4-二酮，六氢-3-（2-甲基丙基）-（PPDHMP）和Didemnin B.根据16S rRNA基因测序和随后的系统进化树分析，该分离株的系统进化分类学表明该分离株是葡萄球菌VITURAJ10（GenBank登录号KX770743.1）的最近邻居。通过MTT测定进一步评估级分对MCF-7细胞系的细胞毒活性。通过GC-MS和FT-IR分析鉴定出的馏分中具有生物学意义的化合物为放线菌素D，吡咯罗[1,2α]吡嗪-1,4-二酮，六氢-3-（2-甲基丙基）-（PPDHMP）和Didemnin B.根据16S rRNA基因测序和随后的系统进化树分析，该分离株的系统进化分类学表明该分离株是葡萄球菌VITURAJ10（GenBank登录号KX770743.1）的最近邻居。