当前位置: X-MOL 学术Antonie van Leeuwenhoek › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Pseudomonas atacamensis sp. nov., isolated from the rhizosphere of desert bloom plant in the region of Atacama, Chile.
Antonie van Leeuwenhoek ( IF 2.6 ) Pub Date : 2020-05-20 , DOI: 10.1007/s10482-020-01427-0
Matías Poblete-Morales 1 , Denisse Carvajal 1, 2 , Romina Almasia 2 , Sebatian Michea 3 , Carolina Cantillana 4 , Arturo Levican 4 , Evelyn Silva-Moreno 1, 5
Affiliation  

The bacterial strain M7D1T was isolated from samples of the rhizosphere of desert bloom plants on the Atacama region located in northern Chile as part of a study intended to isolate nitrifying bacteria in this adverse environment. It was previously identified as belonging to the Pseudomonas fluorescens group. In this study, the phylogenetic analysis of the 16s RNA, gyrA, rpoB and rpoD genes confirmed that this strain belongs to this group, especially Sub Group (SG) Koreensis, but it represents a potential new species. Additionally, the average nucleotide identity confirmed this as the highest identity value (0.92) with Pseudomonas moraviensis LMG 24280, which is lower than the 0.94 threshold established to classify two strains within the same species. The strain M7D1T shared a similar fatty acids methyl ester profile than the type strains of other Pseudomonas spp. previously described. Furthermore, it can be differentiated phenotypically from other related species of SG P. koreensis. Based on these results, the existence of a new species of Pseudomonas is demonstrated, for which the name Pseudomonas atacamensis is proposed. This strain presented a set of genes associated with plant growth-promoting rhizobacteria and it is a good candidate to be used for recovery of contaminated soils. However, more studies are required to demonstrate whether this bacterium is non-pathogenic, can survive in the presence of toxic compounds and promote growth or help to the stress management of plants.



中文翻译:

atacamensis sp。假单胞菌 十一月。,与智利阿塔卡马地区沙漠开花植物的根际隔离。

细菌菌株M7D1 T是从位于智利北部阿塔卡马地区的沙漠开花植物根际样品中分离出来的,作为旨在在这种不利环境中分离硝化细菌的研究的一部分。先前已确定其属于荧光假单胞菌组。在这项研究中,对16s RNA,gyrArpoBrpoD基因的系统发育分析证实,该菌株属于该组,尤其是Koreensis亚组(SG),但它代表了一个潜在的新物种。此外,平均核苷酸同一性证实了它与摩氏假单胞菌的最高同一性值(0.92)LMG 24280,该阈值低于为对同一物种内的两个菌株进行分类而建立的0.94阈值。M7D1 T菌株与其他假单胞菌属的菌株具有相似的脂肪酸甲酯分布。如前所述。此外,它可以在表型上与红皮拟南芥的其他相关物种区分开。根据这些结果,证明存在一种新的假单胞菌属,其名称为Pseudomonas atacamensis被提议。该菌株提供了一组与促进植物生长的根际细菌相关的基因,是用于恢复受污染土壤的良好候选者。但是,需要更多的研究来证明这种细菌是否是非致病性的,是否可以在有毒化合物存在下存活并促进生长或有助于植物的压力管理。

更新日期:2020-07-24
down
wechat
bug