A total of 1,337 serum and plasma specimens (939, 393 and 15 from cattle, sheep and goats, respectively) were collected monthly for one a year from ruminant species slaughtered in three Turkish cities endemic for Crimean-Congo hemorrhagic fever virus (CCHFV), Samsun, Sivas and Tokat. The serum samples were tested by commercial indirect ELISA to detect CCHFV antibodies, and positive or equivocal samples were later confirmed by a virus neutralization test (VNT). The seroprevalence in cattle, sheep, and goats was 36.21% (340/939), 6.27% (24/383), and 6.67% (1/15), respectively. Quantitative real-time RT-PCR was employed to detect viraemic animals at slaughter time. The percentage of CCHFV-viraemic animals was 0.67% (9/1337). The virus load varied between 4.1 x 10¹ and 2.4 x 10³ RNA equivalent copies/mL in viraemic animals. The plasma samples that were positive for CCHFV genomic RNA were collected between April and May, when Hyalomma ticks are active. This study presents quantitative CCHFV load data in ruminant species at slaughter and interprets the likelihood of transmission for employees working in slaughterhouses in CCHFV-endemic regions.

每年从土耳其三个克里米亚-刚果出血热病毒（CCHFV）特有的城市屠宰的反刍动物中，每月收集总计1337份血清和血浆标本（分别来自牛，绵羊和山羊的939、393和15个），一年， Samsun，Sivas和Tokat。通过商业间接ELISA测试血清样品以检测CCHFV抗体，随后通过病毒中和测试（VNT）确认阳性或含糊样品。牛，绵羊和山羊的血清阳性率分别为36.21％（340/939），6.27％（24/383）和6.67％（1/15）。实时定量RT-PCR用于在屠宰时检测病毒动物。CCHFV病毒感染动物的百分比为0.67％（9/1337）。病毒载量在4.1 x 10 ¹和2.4 x 10 ^{3之间变化}病毒感染动物的RNA当量拷贝数/ mL。透明质酸壁虱活跃时，在4月至5月之间收集CCHFV基因组RNA阳性的血浆样品。这项研究提供了在屠宰场中反刍动物中CCHFV数量的定量数据，并解释了CCHFV流行地区在屠宰场工作的员工传播的可能性。