Abstract

A comparative analysis of enzyme-linked immunosorbent assay (ELISA) and quadrupole time-of-flight mass spectrometry (LC-QTOF) for the detection of opioids in blood samples is presented. The Orange County Crime Lab (OCCL) was concerned that the opioid drug class was not accurately detected at low concentrations due to the use of LC-QTOF as a non-targeted screening method for multiple classes of drugs. In order to investigate this issue, 968 ante-mortem and postmortem blood samples were analyzed by ELISA for the presence of the following opioids: morphine, morphine-glucuronide, codeine, codeine-glucuronide, hydrocodone, hydromorphone, hydromorphone-glucuronide, oxycodone, oxymorphone and oxymorphone-glucuronide. All samples had been previously analyzed by LC-QTOF. Overall, 84 samples tested positive for opioids. Discrepant samples between ELISA and LC-QTOF were analyzed by a liquid chromatography tandem mass spectrometry confirmation method in order to determine the true composition of the sample. Upon review of the discrepant samples, no forensically relevant concentration of opioids was missed by LC-QTOF. Thus, the ability of the OCCL’s LC-QTOF screening method was verified to detect opioids at low concentrations.

中文翻译：

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两种芬太尼免疫测定对液相色谱-串联质谱法的性能

摘要

介绍了酶联免疫吸附测定（ELISA）和四极杆飞行时间质谱（LC-QTOF）用于检测血液样品中阿片类药物的比较分析。橙县犯罪实验室（OCCL）担心，由于使用LC-QTOF作为多种药物的非靶向筛选方法，因此在低浓度下无法准确检测出阿片类药物。为了调查此问题，通过ELISA分析了968个死前和死后血样中是否存在以下阿片类药物：吗啡，吗啡-葡糖醛酸，可待因，可待因-葡糖醛酸，氢可酮，氢吗啡酮，氢吗啡酮-葡糖醛酸，羟考酮，羟吗啡酮和羟吗啡酮-葡萄糖醛酸。所有样品之前均已通过LC-QTOF分析。总体而言，有84个样品的阿片类药物检测呈阳性。通过液相色谱串联质谱确认方法分析ELISA和LC-QTOF之间的差异样品，以确定样品的真实组成。在对差异样本进行审查后，LC-QTOF并未遗漏任何与法医有关的浓度。因此，验证了OCCL的LC-QTOF筛选方法能够检测低浓度的阿片类药物。