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New Method of Integrated Photofluorescence Microbiotesting.
Doklady Biological Sciences Pub Date : 2020-03-04 , DOI: 10.1134/s0012496619060103
V S Sibirtsev 1 , A V Garabadgiu 2 , V I Shvets 2, 3
Affiliation  

A biotesting procedure that makes it possible to record the changes in the intensities of elastic light scattering, light absorption, and intrinsic photofluorescence of the protein component, as well as the determination of the concentration and structuring coefficients of the genomic component, of the samples with viable unicellular test organisms incubated in a liquid nutrient medium in the presence and absence of various external chemical factors, is described. The results of the analysis of the antibiotic activity of various metal cations with the use of this technique are presented. This technique is much more rapid, objective, and comprehensive for the assessment of the effect on the reproduction rate, metabolic activity, and genome structure of the test organisms from the samples of various products, wastes, etc. than standard visual microbiotesting methods.

中文翻译:

集成光荧光微生物检测的新方法。

一种生物测试程序,可以记录蛋白质组分的弹性光散射强度,光吸收强度和蛋白质组分的固有光致荧光强度的变化,以及确定基因组组分的浓度和结构系数描述了在存在和不存在各种外部化学因素的情况下,在液体营养培养基中孵育的可生存的单细胞测试生物。给出了使用该技术分析各种金属阳离子的抗生素活性的结果。该技术更加快速,客观和全面,可以评估各种产品,废物等样品对测试生物的繁殖率,代谢活性和基因组结构的影响。
更新日期:2020-03-04
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