Both epithelium and stroma are involved in breast cancer invasion and metastasis. This study aimed at identifying the roles of the stroma in breast cancer tumorigenesis and metastasis. Gene expression profiling GSE10797 was downloaded from the Gene Expression Omnibus database, and it included 28-paired stroma and epithelium breast tissue samples from invasive breast cancer patients and 10 paired normal breast tissue samples. Differentially expressed genes (DEGs) between breast cancer and normal breast tissue samples were identified by using the limma package followed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses to seek the potential functions of DEGs. Moreover, a protein–protein interaction network was constructed based on the String database, and modules were selected through the BioNet tool. Further, functional annotations of DEGs were carried out by using tumor suppressor gene and tumor associated gene databases. Ultimately, KEGG pathway enrichment analysis for DEGs in modules was performed. A total of 38 and 156 DEGs were identified from normal invasive stromal cells and epithelial cells, respectively. DEGs in stromal and epithelial cells were significantly enriched in extracellular matrix (ECM)- and cell proliferation-related functions. COL1A2, a hub node in the stromal module, was mainly enriched in ECM-receptor interaction and focal adhesion pathways. JUN, a hub node in the epithelium module, was significantly enriched in cancer and ErbB signaling pathways. COL1A2, COL1A1, COL3A1, JUN, and FN1 might be vital for tumorigenesis and metastasis of invasive breast cancer. These genes might be potential therapeutic targets for breast cancer treatment.

中文翻译：

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浸润性乳腺癌发生和转移中基质和上皮细胞间差异表达基因的微阵列分析。

上皮细胞和基质都参与了乳腺癌的侵袭和转移。本研究旨在确定基质在乳腺癌肿瘤发生和转移中的作用。基因表达谱 GSE10797 是从 Gene Expression Omnibus 数据库下载的，它包括来自浸润性乳腺癌患者的 28 对基质和上皮乳腺组织样本和 10 对正常乳腺组织样本。乳腺癌和正常乳腺组织样本之间的差异表达基因 (DEG) 通过使用 limma 包进行鉴定，然后是 Gene Ontology 和京都基因和基因组百科全书 (KEGG) 途径富集分析，以寻找 DEG 的潜在功能。此外，一种蛋白质——基于String数据库构建蛋白质相互作用网络，通过BioNet工具选择模块。此外，利用抑癌基因和肿瘤相关基因数据库对DEGs进行功能注释。最终，对模块中的 DEG 进行了 KEGG 通路富集分析。分别从正常侵袭性基质细胞和上皮细胞中鉴定出总共 38 个和 156 个 DEG。基质和上皮细胞中的 DEG 显着富集细胞外基质 (ECM) 和细胞增殖相关功能。COL1A2 是基质模块中的一个枢纽节点，主要富含 ECM-受体相互作用和粘着斑通路。JUN 是上皮模块中的枢纽节点，在癌症和 ErbB 信号通路中显着富集。COL1A2 ,COL1A1、COL3A1、JUN和FN1可能对浸润性乳腺癌的肿瘤发生和转移至关重要。这些基因可能是乳腺癌治疗的潜在治疗靶点。