Hydrogels are used in a wide range of biomedical applications, including three-dimensional (3D) cell culture, cell therapy and bioprinting. To enable processing using advanced additive fabrication techniques and to mimic the dynamic nature of the extracellular matrix (ECM), the properties of the hydrogels must be possible to tailor and change over time with high precision. The design of hydrogels that are both structurally and functionally dynamic, while providing necessary mechanical support is challenging using conventional synthesis techniques. Here, we show a modular and 3D printable hydrogel system that combines a robust but tunable covalent bioorthogonal cross-linking strategy with specific peptide-folding mediated interactions for dynamic modulation of cross-linking and functionalization. The hyaluronan-based hydrogels were covalently cross-linked by strain-promoted alkyne-azide cycloaddition using multi-arm poly(ethylene glycol). In addition, a de novo designed he...

中文翻译：

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动态肽折叠介导的4D生物打印水凝胶的生物功能化和调节。

水凝胶被广泛用于生物医学应用，包括三维（3D）细胞培养，细胞治疗和生物打印。为了能够使用先进的增材制造技术进行加工并模仿细胞外基质（ECM）的动态特性，水凝胶的特性必须能够以高精度调整并随时间变化。使用常规合成技术，在提供必要的机械支持的同时，结构和功能上动态的水凝胶的设计具有挑战性。在这里，我们展示了一个模块化的3D可打印水凝胶系统，该系统将健壮但可调的共价生物正交交联策略与特定的肽折叠介导的相互作用进行了动态调节，以实现交联和功能化的动态调节。透明质酸基水凝胶通过使用多臂聚乙二醇的应变促进炔-叠氮化物环加成进行共价交联。此外，从头设计了...