Abstract

Introduction

Epigenetic alterations in pathogenesis of systemic lupus erythematosus (SLE) have gained more attention recently in adults. We assessed the methylation of CD70 promoter, a costimulatory molecule on T cells, in juvenile SLE (JSLE), and compared this to that found in controls and the literature of adult SLE patients.

Methods

DNA methylation status was evaluated on peripheral blood from JSLE patients and healthy controls.

Results

Twenty-five patients with JSLE and 24 healthy controls were compared. JSLE patients had lower unmethylated CpG islands compared to the control group (mean ± SD; 0.78 ± 0.42 vs 10503.80 ± 39796.95). However, the difference was not significant (P-value; 0.22).

Conclusion

Despite hypomethylation of CD70 gene promoter in CD4+ T-cells from adult patients with SLE, no statistically significant differences observed in patients with JSLE compared with healthy controls. This may suggest a mechanism different in JSLE patients than in adults.

中文翻译：

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幼年系统性红斑狼疮CD70启动子DNA甲基化

摘要

介绍

系统性红斑狼疮 (SLE) 发病机制的表观遗传改变最近在成人中获得了更多关注。我们评估了青少年 SLE (JSLE) 中 CD70 启动子（一种 T 细胞上的共刺激分子）的甲基化，并将其与对照和成人 SLE 患者文献中发现的结果进行了比较。

方法

对来自 JSLE 患者和健康对照者的外周血进行 DNA 甲基化状态评估。

结果

比较了 25 名 JSLE 患者和 24 名健康对照。与对照组相比，JSLE 患者的未甲基化 CpG 岛较低（平均值 ± SD；0.78 ± 0.42 对 10503.80 ± 39796.95）。然而，差异并不显着（P 值；0.22）。

结论

尽管成年 SLE 患者的 CD4+ T 细胞中 CD70 基因启动子低甲基化，但与健康对照组相比，JSLE 患者没有观察到统计学上的显着差异。这可能表明 JSLE 患者的机制与成人不同。