Many persons with diabetes mellitus have limb ischemia, which is a major clinical problem. A subset of human monocytes that expresses TIE-2 may enhance neovascularization. We performed 179 phlebotomies on 142 patients (or donors), including 61 patients/donors without diabetes or ischemia (controls), 39 diabetic nonischemic patients (controls), and 42 diabetic patients with severe limb ischemia requiring amputation. We compared these groups for the presence of TIE-2-positive proangiogenic monocytes. The proportion of proangiogenic monocytes in the venous blood (on hospital admission) was significantly increased in diabetic patients without ischemia (9.22% ± 1.19%), compared to controls (6.53% ± 0.58%) or ischemic diabetic patients (5.44% ± 0.56%) (P < 0.05). In this pilot evaluation, we succeeded in extracting potential proangiogenic TIE-2 monocytes from the blood of diabetic patients without ischemia, but less in patients with ischemia. The implications for therapeutic neoangiogenesis require further studies.

中文翻译：

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慢性缺血性糖尿病足溃疡患者和对照组血液中促血管生成单核细胞的特征。

许多糖尿病患者有肢体缺血，这是一个主要的临床问题。表达 TIE-2 的人类单核细胞亚群可能会增强新血管形成。我们对 142 名患者（或供者）进行了 179 次采血，包括 61 名无糖尿病或缺血的患者/供者（对照）、39 名糖尿病非缺血性患者（对照）和 42 名需要截肢的严重肢体缺血的糖尿病患者。我们比较了这些组是否存在 TIE-2 阳性促血管生成单核细胞。与对照组 (6.53% ± 0.58%) 或缺血性糖尿病患者 (5.44% ± 0.56%) 相比，非缺血性糖尿病患者 (入院时) 静脉血中促血管生成单核细胞的比例显着增加 (9.22% ± 1.19%) ) ( P < 0.05)。在该试点评估中，我们成功地从非缺血糖尿病患者的血液中提取了潜在的促血管生成 TIE-2 单核细胞，但在缺血患者的血液中提取较少。对治疗性新血管生成的影响需要进一步研究。