Multiphoton fluorescence lifetime microscopy has revolutionized studies of pathophysiological and xenobiotic dynamics, enabling the spatial and temporal quantification of these processes in intact organs in vivo . We have previously used multiphoton fluorescence lifetime microscopy to characterise the morphology and amplitude weighted mean fluorescence lifetime of the endogenous fluorescent metabolic cofactor nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) of mouse livers in vivo following induction of various disease states. Here, we extend the characterisation of liver disease models by using nonlinear regression to estimate the unbound, bound fluorescence lifetimes for NAD(P)H, flavin adenine dinucleotide (FAD), along with metabolic ratios and examine the impact of using multiple segmentation methods. We found that NAD(P)H amplitude ratio, and fluorescence lifetime redox ratio can be used as discriminators of diseased liver from normal liver. The redox ra...

中文翻译：

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使用体内多光子荧光寿命成像来揭示肝脏氧化还原状态下特定于疾病的变化。

多光子荧光寿命显微镜已彻底改变了病理生理学和异种生物动力学的研究，使体内完整器官中这些过程的时空定量化成为可能。我们以前曾使用多光子荧光寿命显微镜来表征小鼠体内各种疾病状态诱导后的内源性荧光代谢辅因子烟酰胺腺嘌呤二核苷酸（磷酸盐）（NAD（P）H）的形态和振幅加权平均荧光寿命。在这里，我们通过使用非线性回归估计NAD（P）H，黄素腺嘌呤二核苷酸（FAD）的未结合束缚荧光寿命以及代谢率，扩展了肝脏疾病模型的表征，并研究了使用多种分割方法的影响。我们发现，NAD（P）H振幅比和荧光寿命氧化还原比可以用作正常肝脏患病肝脏的判别指标。氧化还原