The diverse, evolutionary architectures of proteins can be regarded as molecular fossils, tracing a historical path that marks important milestones across life. The B1-subfamily of GPCRs (G-protein-coupled receptors) are medically significant proteins that comprise 15 transmembrane receptor proteins in Homo sapiens. These proteins control the intracellular concentration of cyclic AMP as well as various vital processes in the body. However, little is known about the evolutionary correlation and conservational blueprint of this GPCR subfamily. We performed a comprehensive analysis to understand the evolutionary architecture among 13 members of the B1-subfamily. Multiple sequence alignment analysis exhibited six multiple sequence aligned blocks and five highly aligned blocks. Molecular phylogenetics indicated that CRHR1 and CRHR2 share a typical ancestral relationship and are siblings in 100% bootstrap replications with a total of 24 nodes observed in the cladogram. CRHR2 has the maximum number of extremely conserved amino acids followed by ADCYAP1R1. The longest continuous number sequence logos (74) were found between sequence location 349 and 423, and consequently, the maximum and minimum logo height recorded was 3.6 bits and 0.18 bits, respectively. Finally, to understand the model and pattern of evolutionary relatedness, the conservation blueprint, and the diversification among the members of a protein family, GPCR distribution from several species throughout the animal kingdom was analysed. Together, the study provides an evolutionary insight and offers a rapid method to explore the potential of depicting the evolutionary relationship, conservation blueprint, and diversification among the B1-subfamily of GPCRs using bioinformatics, algorithm analysis, and mathematical models.

中文翻译：

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深入了解 GPCR 的 B1 亚家族成员的进化和保护模式。

蛋白质的多样化、进化结构可以被视为分子化石，追溯着标志着生命中重要里程碑的历史路径。GPCR 的 B1 亚家族（G 蛋白偶联受体）是具有医学意义的蛋白质，在智人中包含 15 种跨膜受体蛋白。这些蛋白质控制环 AMP 的细胞内浓度以及体内的各种重要过程。然而，关于这个 GPCR 亚科的进化相关性和保护蓝图知之甚少。我们进行了全面分析，以了解 B1 亚科 13 个成员的进化结构。多序列比对分析显示六个多序列比对块和五个高度比对块。分子系统发育学表明 CRHR1 和 CRHR2 具有典型的祖先关系，并且在 100% 引导复制中是兄弟姐妹，在进化枝图中观察到总共 24 个节点。CRHR2 具有最大数量的极其保守的氨基酸，其次是 ADCYAP1R1。在序列位置 349 和 423 之间发现了最长的连续数字序列标识（74 个），因此，记录的最大和最小标识高度分别为 3.6 位和 0.18 位。最后，为了了解进化相关性的模型和模式、保护蓝图以及蛋白质家族成员之间的多样化，分析了整个动物王国中几个物种的 GPCR 分布。一起，