This study describes a kinetic spectrophotometric method for accurate, sensitive and rapid determination of cisplatin in biofluids. The developed method is based on the inhibitory effect of cisplatin on the oxidization of Janus Green by bromate in acidic media. The change in absorbance as the criteria of the oxidation reaction was followed spectrophotometrically. To obtain the highest rate of sensitivity, efficient reaction parameters were optimized. Under optimum experimental conditions, a calibration graph was obtained linearly over the range 10.0 – 5750.0 μg L⁻¹ and the limit of detection (3s_b/m) was 4.2 μg L⁻¹ of cisplatin. The interfering effect of diverse species was investigated. The developed method was used for the quantification of cisplatin in bio fluids of patients treated with cisplatin, spiked bio fluids and pharmaceutical samples and yielded satisfactory results.

本研究描述了一种动力学分光光度法，可准确、灵敏且快速地测定生物液中的顺铂。该方法基于顺铂在酸性介质中对溴酸盐氧化 Janus Green 的抑制作用。通过分光光度法跟踪吸光度的变化作为氧化反应的标准。为了获得最高的灵敏度，优化了有效的反应参数。^{在最佳实验条件下，在10.0 – 5750.0 μg L -1}范围内获得线性校准图，顺铂的检测限（3 s _b / m）为4.2 μg L ^{-1 。}研究了不同物种的干扰效应。该方法用于对接受顺铂治疗的患者的生物体液、加标生物体液和药物样品中顺铂的定量，取得了满意的结果。