The aim of this study is to analyze the relationship between Hepatocyte Growth Factor (HGF) levels in oral rinses using water and clinical parameters of periodontitis; and furthermore, to evaluate the potential of a prototype HGF immunochromatographic paper test strip (HGF-TS) for screening of periodontitis, in comparison with a commercially-available occult blood (hemoglobin) test strip (Hb-TS). Clinical periodontal parameters were recorded, and oral rinses were collected, from 125 subjects. Then, the presence of HGF, and hemoglobin (Hb), in each sample was detected using a prototype HGF-TS and an Hb-TS. In addition, the concentrations of HGF and Hb were also determined in each sample is necessary HGF concentrations in oral rinses showed significant correlations with clinical parameters of periodontitis. The positive rate and read value on HGF-TS showed significantly high values in cases of severe periodontitis compared to healthy subjects. Hb-TS showed generally higher positive rates than HGF-TS; however, it showed false positive results in healthy subjects. The concentration of HGF in oral rinses showed close association with the severity of periodontitis, suggesting that the prototype HGF-TS has potential for use in the diagnosis of periodontitis, although further refinement of the test strip is required to increase the sensitivity.

本研究的目的是分析用水冲洗口腔冲洗液中肝细胞生长因子（HGF）水平与牙周炎临床参数之间的关系。此外，与市售的隐性血液（血红蛋白）测试条（Hb-TS）相比，评估原型HGF免疫层析纸测试条（HGF-TS）在筛查牙周炎方面的潜力。记录了125位受试者的临床牙周参数，并收集了漱口水。然后，使用原型HGF-TS和Hb-TS检测每个样品中HGF和血红蛋白（Hb）的存在。此外，还测定了每个样品中HGF和Hb的浓度，这是口腔冲洗液中必需的HGF浓度与牙周炎的临床参数之间存在显着相关性。与健康受试者相比，严重牙周炎病例中HGF-TS的阳性率和读取值显示出很高的值。Hb-TS的阳性率普遍高于HGF-TS；但是，它在健康受试者中显示出假阳性结果。口腔冲洗液中HGF的浓度与牙周炎的严重程度密切相关，这表明原型HGF-TS具有用于牙周炎诊断的潜力，尽管需要进一步完善试纸以提高敏感性。