Comparing phytoplankton non-bloom and bloom communities using rRNA and its coding gene can help understand the shift of dominant species and its driving processes (e.g., intrinsic growth or grazing). Here we conducted high-throughput sequencing of 18S rRNA and its coding gene for studying non-bloom and bloom plankton communities in East China Sea. The non-bloom community was dominated by diatoms whereas during the bloom it was dominated by the dinoflagellate Prorocentrum shikokuense (formerly P. donghaiense). P. shikokuense rRNA abundance and rRNA:rRNA gene ratio both increased markedly in the bloom, indicating that the bloom arose from active growth. In contrast, some non-bloom species showed low DNA abundances during the bloom albeit high rRNA:rRNA gene ratios, suggesting that cell loss processes such as grazing might have prevented these species from blooming or that these species might be at an early stage of bloom development. Furthermore, Pearson's correlation analysis showed that dinoflagellate abundance was positively correlated with temperature and negatively related to dissolved inorganic phosphate (DIP) concentrations, suggesting warm and DIP-poor environment as a niche space for P. shikokuense. Our results demonstrate the usefulness of combined analysis of rRNA and its gene in characterizing phytoplankton bloom development to shed light on the complex phytoplankton dynamics and regulating mechanisms in the course of bloom development.

使用rRNA及其编码基因比较浮游植物的非开花和开花群落，可以帮助了解优势物种的迁移及其驱动过程（例如内在生长或放牧）。在这里，我们进行了18S rRNA及其编码基因的高通量测序，以研究东海的非开花和开花浮游生物群落。非开花群落以硅藻为主，而在开花期则以鞭毛原螯虾（Prorocentrum shikokuense）（以前为东海参）为主。石shrRNA丰度和rRNA：rRNA基因比率在花期均显着增加，表明花期是由活跃的生长而产生的。相比之下，尽管rRNA：rRNA基因比例高，但一些非盛开物种在盛开期间显示出较低的DNA丰度，这表明诸如放牧等细胞丢失过程可能阻止了这些物种盛开，或者这些物种可能处于盛开的早期。发展。此外，Pearson的相关分析表明，鞭毛含量与温度呈正相关，与溶解的无机磷酸盐（DIP）浓度呈负相关，这表明温暖而DIP贫乏的环境是Shikokuense的利基空间。。我们的结果表明，rRNA及其基因的组合分析可用于表征浮游植物的水华发育，从而阐明水华发展过程中复杂的浮游植物动力学和调控机制。