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Limnospira indica PCC8005 growth in photobioreactor: model and simulation of the ISS and ground experiments.
Life Sciences in Space Research ( IF 2.5 ) Pub Date : 2020-03-13 , DOI: 10.1016/j.lssr.2020.03.002
Laurent Poughon 1 , Céline Laroche 1 , Catherine Creuly 1 , Claude-Gilles Dussap 1 , Christel Paille 2 , Christophe Lasseur 2 , Pieter Monsieurs 3 , Wietse Heylen 3 , Ilse Coninx 3 , Felice Mastroleo 3 , Natalie Leys 3
Affiliation  

The Arthrospira-B experiment is the first experiment in space ever allowing the online measurements of both oxygen production rate and growth rate of Limnospira indica PCC8005 in batch photobioreactors running on-board ISS. Four bioreactors were integrated in the ISS Biolab facility. Each reactor was composed of two chambers (gas and liquid) separated by a PTFE membrane and was run in batch conditions. Oxygen production was monitored by online measurement of the total pressure increase in the gas chamber. The experiments are composed of several successive batch cultures for each reactor, performed in parallel on ISS and on ground. In this work, a model for the growth of the cyanobacterium Limnospira indica PCC8005 (also known as Arthrospira or spirulina) in these space membrane photobioreactors was proposed and the simulation results obtained are compared to the experimental results gathered in space and on ground.

The photobioreactor model was based on a light transfer limitation model, already used to describe and predict the growth and oxygen production in small to large scale ground photobioreactors. It was completed by a model for pH prediction in the liquid phase allowing assessment of the pH increase associated to the bicarbonate consumption for the biomass growth. A membrane gas-liquid transfer model is used to predict the gas pressure increase in the gas chamber. Substrate limitation is considered in the biological model.

A quite satisfactory fit was achieved between experimental and simulation results when a suitable mixing of the liquid phase was maintained. The data showed that microgravity has no first order effect on the oxygen production rate of Limnospira indica PCC8005 in a photobioreactor operating in space in zero gravity conditions.



中文翻译:

Limnospira indica PCC8005在光生物反应器中的生长:ISS的模型和仿真以及地面实验。

Arthrospira-B实验是有史以来第一个允许在线测量在ISS上运行的间歇式光生物反应器中的Limnospira indica PCC8005的产氧率和生长率的太空实验。四个生物反应器已整合到ISS Biolab工厂中。每个反应器由被PTFE膜隔开的两个腔室(气体和液体)组成,并在间歇条件下运行。通过在线测量气室中总压力的增加来监测氧气的产生。实验由每个反应器的几个连续分批培养组成,在ISS和地面上并行进行。在这项工作中,对于蓝藻的生长模型Limnospira籼稻PCC8005(也被称为节旋 在这些空间膜光生物反应器中提出了螺旋藻或螺旋藻),并将获得的模拟结果与在空间和地面上收集的实验结果进行了比较。

光生物反应器模型基于光传输限制模型,该模型已经用于描述和预测小规模到大型地面光生物反应器的生长和氧气产生。它由用于液相中pH预测的模型完成,从而可以评估与生物质生长的碳酸氢盐消耗量相关的pH升高。膜气液传递模型用于预测气室中的气压增加。在生物模型中考虑了底物限制。

当保持液相的适当混合时,在实验结果和模拟结果之间获得了非常令人满意的拟合。数据表明,微重力对在零重力条件下在太空中运行的光生物反应器中的印度小螺螺PCC8005的产氧率没有一级影响。

更新日期:2020-03-13
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