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Dietary Fat Does Not Overcome trans-10, cis-12 Conjugated Linoleic Acid Inhibition of Milk Fat Synthesis in Lactating mice.
Lipids ( IF 1.9 ) Pub Date : 2020-02-24 , DOI: 10.1002/lipd.12228
Megan M Robblee 1 , Yves R Boisclair 1 , Dale E Bauman 1 , Kevin J Harvatine 1, 2
Affiliation  

Trans‐10, cis‐12 conjugated linoleic acid (CLA) is a potent inhibitor of milk fat synthesis in the cow and similarly reduces milk fat in rodents. The objective of this study was to determine whether dietary fat can overcome CLA inhibition of milk fat concentration in lactating mice. Wild type C57Bl/6J mice (n = 31) were fed semipurified diets containing either low fat (LF; 4% fat) or high fat (HF; 23.6% fat) starting 4–6 days postpartum. Dietary fat was increased by inclusion of high oleic sunflower oil. After 2 days on the experimental diets, lactating dams were orally dosed with either water (control) or trans‐10, cis‐12 CLA (20 mg/day) for 5 days. CLA treatment decreased pup growth similarly in both HF and LF diets. Milk fat percent was increased over 16% by the HF diet and decreased over 12% by CLA, but there was no interaction of dietary fat and CLA. Both CLA and the HF diet reduced the proportion of short‐ and medium‐chain fatty acids that originate from de novo synthesis, and there was no interaction of diet and CLA. CLA had no effect on the percent of preformed fatty acids, but the HF diet increased their abundance. Dietary fat and CLA both modified mammary expression of lipogenic enzymes and regulators, but no interactions were observed. In conclusion, CLA reduced milk fat concentration and litter growth, but these effects were not overcome by increased dietary fat from high oleic sunflower oil. CLA inhibition of milk fat in the mammary gland is not substrate dependent, and the mechanism is independent from dietary supply of oleic acid.

中文翻译:

饮食中的脂肪不能克服乳鼠脂肪合成中的反式10,顺式12共轭亚油酸抑制作用。

反式10,顺式12共轭亚油酸(CLA)是奶牛体内乳脂合成的有效抑制剂,并且同样减少了啮齿动物的乳脂。这项研究的目的是确定饮食中的脂肪是否可以克服CLA对泌乳小鼠乳脂浓度的抑制作用。从产后4-6天开始,对野生型C57Bl / 6J小鼠(n = 31)进行半纯化饮食,该饮食含有低脂(LF; 4%脂肪)或高脂(HF; 23.6%脂肪)。食用高油酸葵花籽油可增加膳食脂肪。在实验饮食的两天后,给哺乳期母鼠口服水(对照)或反式-10,顺式‐12 CLA(20 mg /天),持续5天。在高频和低频饮食中,CLA处理均会降低幼犬的生长。HF饮食使乳脂百分比增加了16%以上,而CLA则使乳脂百分比减少了12%以上,但是饮食脂肪和CLA之间没有相互作用。CLA和HF饮食均降低了从头产生的短链和中链脂肪酸的比例合成,饮食和CLA之间没有相互作用。CLA对预先形成的脂肪酸的百分比没有影响,但是HF饮食增加了它们的丰度。膳食脂肪和CLA均能改变脂肪酶和调节剂的乳腺表达,但未观察到相互作用。总之,CLA降低了乳脂浓度和凋落物的生长,但是高油酸葵花籽油增加饮食脂肪并不能克服这些影响。CLA抑制乳腺中的乳脂不依赖于底物,其机制与饮食中油酸的供应无关。
更新日期:2020-02-24
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