At present, most of the drugs have little effect on the pathological process of rheumatoid arthritis (RA). Analgesia is an important measure in the treatment of RA and is also one of the criteria to determine the therapeutic effects of the disease. Some studies have found that crocin, a kind of Chinese medicine, can effectively alleviate pain sensitization in pain model rats, but the mechanism is not clear. Emerging evidence indicates that crocin may inhibit the metastasis of lung and liver cancer cells from the breast by inhibiting Wnt/β-catenin and the Wnt signaling pathway is closely related to RA. Wnt5a belongs to the Wnt protein family and was previously thought to be involved only in nonclassical Wnt signaling pathways. Recent studies have shown that Wnt5a has both stimulatory and inhibitory effects on the classical Wnt signaling pathway, and so, Wnt5a has attracted increasing attention. This study demonstrated that crocin significantly increased the mechanical thresholds of adjuvant-induced arthritis (AIA) rats, suggesting that crocin can alleviate neuropathic pain. Crocin significantly decreased the levels of pain-related factors and glial activation. Foxy5, activator of Wnt5a, inhibited the above effects of crocin in AIA rats. In addition, intrathecal injection of a Wnt5a inhibitor significantly decreased hyperalgesia in AIA rats. This research shows that crocin may alleviate neuropathic pain in AIA rats by inhibiting the expression of pain-related molecules through the Wnt5a/β-catenin pathway, elucidating the mechanism by which crocin relieves neuropathic pain and provides a new way of thinking for the treatment of AIA pain.

中文翻译：

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番红花通过抑制脊髓Wnt5a /β-Catenin信号传导途径和胶质细胞活化来缓解AIA大鼠疼痛痛觉过敏。

目前，大多数药物对类风湿关节炎（RA）的病理过程影响很小。镇痛是治疗RA的重要措施，也是确定疾病治疗效果的标准之一。一些研究发现，番红花是一种中药，可以有效减轻疼痛模型大鼠的疼痛致敏性，但机理尚不清楚。新兴证据表明，crocro可能通过抑制Wnt / β来抑制乳腺的肺癌和肝癌细胞的转移-catenin和Wnt信号通路与RA密切相关。Wnt5a属于Wnt蛋白家族，以前被认为仅参与非经典Wnt信号传导途径。最近的研究表明，Wnt5a对经典Wnt信号通路具有刺激和抑制作用，因此Wnt5a引起了越来越多的关注。这项研究表明，crocrocin显着提高了佐剂性关节炎（AIA）大鼠的机械阈值，表明crocroin可以减轻神经性疼痛。番红花显着降低了疼痛相关因子和神经胶质激活的水平。Wnt5a的激活剂Foxy5抑制了crocin在AIA大鼠中的上述作用。此外，鞘内注射Wnt5a抑制剂可显着降低AIA大鼠的痛觉过敏。β -catenin途径阐明了番红花减轻神经性疼痛的机制，并为治疗AIA疼痛提供了新的思路。