Background: Semaphorin 3B (SEMA3B) is characterized as a strong suppressing factor of the proliferation of cancerous cells and also by its anti-angiogenic effect. However, the knowledge on the changes in the expression profile of SEMA3B under the influence of cisplatin in endometrial cancer remains fragmented. The aim of this work was to note the changes in expression of SEMA3B when under the influence of cisplatin in the endometrial cancer cell line.

Methods: Ishikawa cell line cells were exposed to three different concentrations of cisplatin: 2.5μM; 5μM; 10μM for 12, 24 and 48 hours and were compared to cells untreated by the drug. Changes in the expression profile of SEMA3B were determined based upon RtqPCR (mRNA) alongside the ELISA assay (protein). The Statistica 13.0 PL program was used for statistical analysis (p<0.05).

Results: Changes on the transcriptome level seem to be more dynamic than on the proteome level. Regardless of the concentration given or the exposition period, the expression of semaphorin 3B was, in fact, higher in cells exposed to cisplatin. Statistically substantial differences (p<0.05) in the expression of SEMA3B mRNA and protein were seen for all incubation periods at the given cisplatin level when compared to the control.

Conclusion: Cisplatin causes a growth in the expression of SEMA3B in an endometrial cancer cell culture, this results in the restoration in the state of cell homeostasis and shows the effectiveness of pharmacotherapy, including a low risk of drug resistance.

背景：Semaphorin 3B（SEMA3B）是癌细胞增殖的强大抑制因子，并具有抗血管生成作用。但是，关于子宫内膜癌中顺铂影响下的SEMA3B表达谱变化的知识仍然是零碎的。这项工作的目的是注意到子宫内膜癌细胞系中顺铂影响下SEMA3B表达的变化。

方法：将石川细胞系细胞暴露于三种不同浓度的顺铂：2.5μM；5微米 10μM持续12、24和48小时，然后与未经药物处理的细胞进行比较。基于RtqPCR（mRNA）和ELISA分析（蛋白质）确定SEMA3B表达谱的变化。使用Statistica 13.0 PL程序进行统计分析（p <0.05）。

结果：转录组水平的变化似乎比蛋白质组水平更动态。无论给定浓度或暴露时间长短，实际上信号量3B在暴露于顺铂的细胞中的表达都较高。与对照相比，在给定的顺铂水平下的所有孵育期间，均观察到SEMA3B mRNA和蛋白质表达的统计学上显着差异（p <0.05）。

结论：顺铂引起子宫内膜癌细胞培养中SEMA3B表达的增加，从而导致细胞稳态的恢复，并显示出药物治疗的有效性，包括低耐药性。