In this study, an ion exchange resin‐based downstream‐processing concept for imine reductase (IRED)‐catalyzed reactions was investigated. As a model reaction, 2‐methylpyrroline was converted to its corresponding product (S)‐2‐methylpyrrolidine with >99% of conversion by the (S)‐selective IRED from Paenibacillus elgii B69. Under optimized reaction conditions full conversion was achieved using a substrate concentration of 150 and 500 mmol/L of d‐glucose. Seven commercially available cation‐ and anion‐exchange resins were studied with respect to their ability to recover the product from the reaction solution. Without any pretreatment, cation‐exchange resins Amberlite IR‐120(H), IRN‐150, Dowex Monosphere 650C, and Dowex Marathon MSC showed high recovery capacities (up to >90%). A 150‐ml preparative scale reaction was performed yielding ~1 g hydrochloride salt product with >99% purity. Any further purification steps, for example, by column chromatography or recrystallization, were not required.

中文翻译：

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用于亚胺还原酶催化反应的下游加工方法的离子交换树脂材料的整合。

在这项研究中，研究了基于离子交换树脂的亚胺还原酶 (IRED) 催化反应的下游加工概念。作为模型反应，2-甲基吡咯啉通过来自类芽孢杆菌B69的（S）选择性IRED转化为其相应的产物（S）-2-甲基吡咯烷，转化率 > 99% 。在优化的反应条件下，使用 150 和 500 mmol/L 的d底物浓度实现了完全转化-葡萄糖。研究了七种市售阳离子和阴离子交换树脂从反应溶液中回收产物的能力。在未经任何预处理的情况下，阳离子交换树脂 Amberlite IR-120(H)、IRN-150、Dowex Monosphere 650C 和 Dowex Marathon MSC 显示出很高的回收能力（高达 >90%）。进行了 150 毫升的制备规模反应，产生了约 1 克纯度 > 99% 的盐酸盐产品。不需要任何进一步的纯化步骤，例如通过柱层析或重结晶。