ABSTRACT: During 1992 and 1993, a bacterial disease occurred in a seawater Atlantic salmon Salmo salar farm, causing serious mortalities. The causative agent was subsequently named as Oceanivirga salmonicida, a member of the Leptotrichiaceae. Searches of 16S rRNA gene sequence databases have shown sequence similarities between O. salmonicida and uncultured bacterial clones from the digestive tracts of marine mammals. In the current study, oral samples were taken from stranded dolphins (common dolphin Delphinus delphis, striped dolphin Stenella coeruleoalba) and healthy harbour seals Phoca vitulina. A bacterium with growth characteristics consistent with O. salmonicida was isolated from a common dolphin. The isolate was confirmed as O. salmonicida, by comparisons to the type strain, using 16S rRNA gene, gyrB, groEL, and recA sequence analyses, average nucleotide identity analysis, and MALDI-TOF mass spectrometry. Metagenomic analysis indicated that the genus Oceanivirga represented a significant component of the oral bacterial microbiomes of the dolphins and seals. However, sequences consistent with O. salmonicida were only found in the dolphin samples. Analyses of marine mammal microbiome studies in the NCBI databases showed sequences consistent with O. salmonicida from the common dolphin, striped dolphin, bottlenose dolphin Tursiops truncatus, humpback whale Megaptera novaeangliae, and harbour seal. Sequences from marine environmental studies in the NCBI databases showed no sequences consistent with O. salmonicida. The findings suggest that several species of marine mammals are natural hosts of O. salmonicida.

中文翻译：

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海洋哺乳动物是大西洋鲑的细菌病原体Oceanivirga salicida的天然宿主。

摘要：在1992年至1993年期间，大西洋大西洋鲑鱼Salmo盐沼养殖场发生细菌性疾病，造成了致命的死亡。病原体随后命名为Oceanivirga鲑，所述的构件Leptotrichiaceae。对16S rRNA基因序列数据库的搜索显示，沙门氏菌和海洋哺乳动物消化道未培养的细菌克隆之间的序列相似性。在当前的研究中，口服样品取自搁浅的海豚（常见的海豚Delphinus delphis，条纹海豚Stenella coeruleoalba）和健康的斑海豹Phoca vitulina。具有与鲑鱼分离自普通海豚。通过与16S rRNA基因，gyrB，groEL和recA序列分析，平均核苷酸同一性分析和MALDI-TOF质谱分析与类型菌株进行比较，确认该分离株为鲑鱼。元基因组学分析表明，Oceanivirga属代表了海豚和海豹口腔细菌微生物组的重要组成部分。但是，仅在海豚样品中发现了与鲑鱼O. salicida一致的序列。在NCBI数据库中对海洋哺乳动物微生物组研究的分析显示，序列与鲑鱼O. salicida一致来自普通的海豚，条纹海豚，宽吻海豚Tursiops truncatus，座头鲸Megaptera novaeangliae和海豹。在NCBI数据库中进行的海洋环境研究的序列显示，没有序列与鲑鱼一致。调查结果表明，海洋哺乳动物的几个种类的天然宿主O.鲑。