The importance of N6-methyladenosine (m6A) in tumor recurrence and prognosis has been recognized in recent years. The role of m6A readers, such as insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2), in regulating head and neck squamous cell carcinoma (HNSCC) remains unclear. We, therefore, assessed the prognostic role of IGF2BP2 in HNSCC using openly available data from The Cancer Genome Atlas (TCGA) in conjunction with HNSCC patient sample immunohistochemistry (n = 36). The correlation between IGF2BP2 expression and clinical characteristics was then examined. The role of IGF2BP2 in prognosis was assessed by Kaplan-Meier curves and Cox analysis. Finally, TCGA data was used to explore possible carcinogenic mechanisms with multi-GSEA (gene set enrichment analysis). Analysis of TCGA data and IHC results revealed that IGF2BP2 was upregulated in HNSCC tumor tissues, and the expression level was related to the T stage. Simultaneously, Kaplan-Meier curves and Cox analysis indicated that highly expressed IGF2BP2 correlated with poor prognosis and that IGF2BP2 was a potential prognostic factor for HNSCC. Gene set enrichment analysis revealed that scavenging and degradation, synthesis and metabolism, cell growth, death and motility, and cancer pathways were differentially enriched in patients with high IGF2BP2 expression. Our results demonstrate that IGF2BP2 plays an important role in tumor progression and may serve as an important biological prognostic factor for HNSCC.

中文翻译：

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m6A阅读器基因IGF2BP2在头颈部鳞状细胞癌中的临床意义。

近年来，已经认识到N6-甲基腺苷（m6A）在肿瘤复发和预后中的重要性。m6A阅读器，如胰岛素样生长因子2 mRNA结合蛋白2（IGF2BP2）在调节头颈部鳞状细胞癌（HNSCC）中的作用尚不清楚。因此，我们结合癌症基因组图谱（TCGA）与HNSCC患者样品免疫组化方法（n = 36），使用公开可用的数据评估了IGF2BP2在HNSCC中的预后作用。然后检查了IGF2BP2表达与临床特征之间的相关性。通过Kaplan-Meier曲线和Cox分析评估了IGF2BP2在预后中的作用。最后，TCGA数据被用于探索具有多种GSEA（基因集富集分析）的可能的致癌机制。TCGA数据和IHC结果的分析表明，IGF2BP2在HNSCC肿瘤组织中上调，其表达水平与T期有关。同时，Kaplan-Meier曲线和Cox分析表明，高表达的IGF2BP2与不良预后相关，而IGF2BP2是HNSCC的潜在预后因素。基因集富集分析显示，在IGF2BP2高表达患者中，清除和降解，合成和代谢，细胞生长，死亡和运动以及癌症途径均差异富集。我们的结果表明，IGF2BP2在肿瘤进展中起着重要作用，并且可以作为HNSCC的重要生物学预后因素。Kaplan-Meier曲线和Cox分析表明，高表达的IGF2BP2与不良预后相关，而IGF2BP2是HNSCC的潜在预后因素。基因集富集分析显示，在IGF2BP2高表达患者中，清除和降解，合成和代谢，细胞生长，死亡和运动以及癌症途径均差异富集。我们的结果表明，IGF2BP2在肿瘤进展中起着重要作用，并且可以作为HNSCC的重要生物学预后因素。Kaplan-Meier曲线和Cox分析表明，高表达的IGF2BP2与不良预后相关，而IGF2BP2是HNSCC的潜在预后因素。基因集富集分析显示，在IGF2BP2高表达患者中，清除和降解，合成和代谢，细胞生长，死亡和运动以及癌症途径均差异富集。我们的结果表明，IGF2BP2在肿瘤进展中起着重要作用，并且可以作为HNSCC的重要生物学预后因素。IGF2BP2高表达的患者中，癌症的途径和差异得到了丰富。我们的结果表明，IGF2BP2在肿瘤进展中起着重要作用，并且可能作为HNSCC的重要生物学预后因素。IGF2BP2高表达的患者中，癌症的途径和差异得到了丰富。我们的结果表明，IGF2BP2在肿瘤进展中起着重要作用，并且可以作为HNSCC的重要生物学预后因素。