Purpose: The present paper investigated the expression of TLR2 in serum of patients with pulmonary tuberculosis and lung cancer, and verifiedthe effect of TLR2 on the biological characteristics of lung cancer cells. Methods: The common differentially expressed genes in tuberculosis and lung cancer samples were analyzed by edgeR. The intersection of genes was taken and the enrichment analysis and string interaction analysis were performed. The expression of TLR2, inflammatory factors IL6, IL17 and IL22 in serum of patients with pulmonary tuberculosis or lung cancer and lung cell were detected by ELISA. The mRNA and protein expression levels of TLR2, caspase-3, Bax and Bcl-2 were detected by qRT-PCR and Western blot. CCK-8, colony formation assay, transwell assay and flow cytometry were performed to detect the proliferation, invasion, migration and cells apoptosis of lung cancer cells. Results: Bioinformatics analysis found that high expression of TLR2 is a core regulator in lung cancer and tuberculosis. TLR2 and inflammatory factors IL6, IL17, IL22 are highly expressed in the serum of patients with tuberculosis and lung cancer by ELISA.TLR2 is also highly expressed in lung cancer cells. Silencing TLR2 inhibited the growth, invasion and migration ability of cells, and the expression of IL6, IL17 and IL22. It also promoted the expression of caspase-3 and Baxwith the decreased expression of Bcl-2. Conclusion: TLR2 and inflammatory factors IL6, IL17 and IL22 were highly expressed in the serum of patients with pulmonary tuberculosis and lung cancer. Silencing TLR2 could inhibit the growth, invasion and migration ability of lung cancer cells, and promote apoptosis.

中文翻译：

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肺结核和肺癌患者血清中TLR2的高表达，可促进肺癌的发展。

目的：研究肺结核和肺癌患者血清中TLR2的表达，并验证TLR2对肺癌细胞生物学特性的影响。方法：通过edgeR分析结核和肺癌样本中常见的差异表达基因。取基因的交集并进行富集分析和字符串相互作用分析。ELISA法检测肺结核或肺癌患者血清和肺细胞中TLR2，炎症因子IL6，IL17和IL22的表达。用qRT-PCR和Western blot检测TLR2，caspase-3，Bax和Bcl-2的mRNA和蛋白表达水平。进行CCK-8，集落形成分析，transwell分析和流式细胞仪检测增殖，侵袭，肺癌细胞的迁移和细胞凋亡。结果：生物信息学分析发现，TLR2的高表达是肺癌和结核病的核心调节因子。通过ELISA，TLR2和炎症因子IL6，IL17，IL22在结核病和肺癌患者的血清中高表达，而TLR2在肺癌细胞中也高表达。沉默TLR2抑制细胞的生长，侵袭和迁移能力以及IL6，IL17和IL22的表达。它也促进了caspase-3和Bax的表达，而Bcl-2的表达却降低了。结论：肺结核和肺癌患者血清中TLR2和炎性因子IL6，IL17和IL22高表达。沉默TLR2可以抑制肺癌细胞的生长，侵袭和迁移能力，并促进细胞凋亡。