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Simultaneous Determination of Prostaglandin and Hormones in Excreta of Trogopterus xanthipes.
Journal of Chromatographic Science ( IF 1.3 ) Pub Date : 2020-05-14 , DOI: 10.1093/chromsci/bmaa017
Haolong Liu 1, 2 , Miaoxuan Fan 2 , Xintong Fu 2 , Yougen Chen 2 , Min Ye 1 , Hongzhu Guo 2
Affiliation  

The excreta of Trogopterus xanthipes (also called Wulingzhi in Chinese, WLZ) is a well-known traditional Chinese medicine used for the treatment of irregular menstruation in clinic. Few reports are available on the chemical profiling of WLZ. In this work, qualitative and quantitative analyses of endogenous prostaglandin and hormones in WLZ were performed using UHPLC-orbitrap-MSn. In total, 48 compounds were identified in urine of T. xanthipes. Furthermore, the contents of four target compounds were simultaneously quantitated in 20 batches of samples by UPLC-MS/MS. The quantitative method showed a good linear correlation (R > 0.995) in a wide range for each compound. The method had a high sensitivity with LOD (0.5–1.0 ng/mL) and LOQ (1.0–2.5 ng/mL). The intra- and inter-day precisions were < 9.17 (RSD %), and repeatability and stability were < 6.14 (RSD %). The recovery of the analytes varied between 85.8% and 97.3% at three different concentrations. The present integrated qualitative and quantitative assessment of WLZ provides an evaluation strategy to assess the constituent in traditional Chinese medicine.

中文翻译:

同时测定黄Tro的排泄物中前列腺素和激素的含量。

Trogopterus xanthipes的排泄物(中文也称为五灵芝,WLZ)是一种著名的中药,用于治疗临床上的月经不调。很少有关于WLZ的化学分析的报告。在这项工作中,使用UHPLC-orbitrap-MS n对WLZ中的内源性前列腺素和激素进行了定性和定量分析。总共在T. xanthipes的尿液中鉴定出48种化合物。此外,通过UPLC-MS / MS在20批样品中同时定量了四种目标化合物的含量。定量方法显示出良好的线性相关性(R > 0.995)的范围,每种化合物都可以。该方法对LOD(0.5–1.0 ng / mL)和LOQ(1.0–2.5 ng / mL)具有高灵敏度。日内和日间精度为<9.17(RSD%),重复性和稳定性为<6.14(RSD%)。在三种不同浓度下,分析物的回收率在85.8%和97.3%之间变化。当前的WLZ定性和定量综合评估为评估中药成分提供了一种评估策略。
更新日期:2020-05-14
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