This study aims to achieve the principles of tissue engineering using biopolymers to be applied in the field of vital endodontic treatment to stimulate stem cells and engineering and regeneration of dentin tissue. the polymer blend was loaded with the steroidal anti-inflammatory drug, dexamethasone, and the porous drug-loaded bio-sponge was produced by lyophilization. Bio-sponge, as a direct pulp capping agent, was histologically studied compared to calcium hydroxide Ca(OH)₂ in an animal experiment. The results indicated the effectiveness of the bio-sponge as a direct pulp capping agent where the dentin bridge was formed faster than Ca(OH)₂ treated samples. There was no inflammatory response in the pulp tissue throughout the follow-up period. The porous bio-sponge loaded with dexamethasone with a neutral pH resulted in enhancement of the odontoblast differentiation from stem cells, resulting in the formation of a renewed dentin bridge without the slightest inflammatory response in the pulp.

本研究旨在实现使用生物聚合物的组织工程原理，应用于重要的牙髓治疗领域，以刺激干细胞和牙本质组织的工程化和再生。聚合物共混物负载甾体抗炎药地塞米松，多孔载药生物海绵通过冻干法制备。生物海绵作为直接盖髓剂，在动物实验中与氢氧化钙 Ca(OH) ₂进行了组织学研究。结果表明生物海绵作为直接盖髓剂的有效性，其中牙本质桥的形成速度比 Ca(OH) ₂快处理过的样品。在整个随访期间，牙髓组织中没有炎症反应。加载有中性 pH 值的地塞米松的多孔生物海绵可增强干细胞向成牙本质细胞的分化，从而形成更新的牙本质桥，而牙髓中没有丝毫的炎症反应。