Bacterial wilt and brown rot disease caused by Ralstonia solanacearum species complex (RSSC) is one of the major constraints of potato (Solanum tuberosum) production around the globe. During 2017 to 2018, an extensive field survey was conducted in six potato-growing provinces of Iran to monitor the status of bacterial wilt disease. Pathogenicity and host range assays using 59 bacterial strains isolated in Iran showed that they were pathogenic on eggplant, red nightshade, pepper, potato and tomato, while nonpathogenic on common bean, cowpea, cucumber, sunflower, zinnia and zucchini. PCR-based diagnosis revealed that the strains belong to the phylotype IIB/sequevar 1 (IIB/I) lineage of the RSSC. Furthermore, a five-gene multilocus sequence analysis and typing (egl, fliC, gyrB, mutS, and rplB) confirmed the phylogenetically near-homogeneous nature of the strains within IIB/I lineage. Four sequence types were identified among 58 IIB/1 strains isolated in Iran. Phylogenetically near-homogeneous nature of the strains in Iran raise questions about the mode of inoculum entry of the bacterial wilt pathogen into the country (one-time introduction versus multiple introductions), while the geographic origin of the Iranian R. solanacearum strains remains undetermined. Furthermore, sequence typing showed that there were shared alleles (haplotypes) and sequence types among the strains isolated in geographically distant areas in Iran, suggesting intranational transmission of the pathogen in the country.

由Ralstonia solanacearum种复合物（RSSC）引起的细菌性枯萎和褐腐病是全球马铃薯（Solanum tuberosum）生产的主要制约因素之一。在2017年至2018年期间，在伊朗的六个马铃薯种植省进行了广泛的实地调查，以监测细菌性枯萎病的状况。使用在伊朗分离的59种细菌菌株进行的致病性和宿主范围分析表明，它们对茄子，红色茄属植物，胡椒，马铃薯和番茄具有致病性，而对普通豆、,豆，黄瓜，向日葵，百日草和西葫芦则无致病性。基于PCR的诊断表明，该菌株属于RSSC的系统型IIB / sequevar 1（IIB / I）谱系。此外，五基因多基因座序列分析和分类（egl，fliC，gyrB，mutS和rplB）证实了IIB / I谱系中菌株的系统发育近均质性。在伊朗分离的58株IIB / 1菌株中鉴定出四种序列类型。伊朗菌株在系统发育上近乎同质，这引发了关于细菌枯萎病原体进入该国家的接种方式（一次性引入与多次引入）的质疑，而伊朗青枯菌的地理起源菌株仍未确定。此外，序列分型显示在伊朗地理上偏远地区分离出的菌株之间存在共享的等位基因（单倍型）和序列类型，表明该病原体在国内传播。