The major troubles in 6-(N-hydroxyethyl)-amino-6-deoxy-α-l-sorbofuranose (6NSL) production from N-2-hydroxyethyl glucamine (NHEG) by Gluconobacter oxydans were low cell yield during cell preparation and loss of cells’ biocatalytic ability during biotransformation, resulting in high production cost and low 6NSL production. The target of this work was to enhance 6NSL production by reusing cells and improving the cells biocatalytic ability. First, inhibitory effects of substrate and product on 6NSL production, and optimization of cell regeneration condition were investigated, respectively. Then repeated production of 6NSL by immobilized cell using a strategy of in situ exhaustive cell regeneration in a bubble column bioreactor was developed. As a result, the bioprocess underwent nine cycles, the average 6NSL production and conversion rate of NHEG to 6NSL reached 42.6 g L⁻¹ and 83.1% in each batch was achieved, respectively.

氧化葡糖杆菌从N -2-羟乙基葡糖胺（NHEG）生产6-（N-羟乙基）-氨基-6-脱氧-α - 1-山梨糖呋喃糖（6NSL）的主要问题在细胞制备过程中细胞产量低，在生物转化过程中细胞生物催化能力的丧失，导致生产成本高和6NSL产量低。这项工作的目标是通过重用细胞并提高细胞的生物催化能力来增强6NSL的产生。首先，分别研究了底物和产物对6NSL产生的抑制作用以及细胞再生条件的优化。然后开发了在气泡柱生物反应器中通过原位穷竭细胞再生策略由固定化细胞重复生产6NSL的方法。结果，生物过程经历了九个循环，每批中平均6NSL产生和NHEG向6NSL的转化率分别达到42.6g L ^-1和83.1％。