Spinal cord injury is a devastating condition of the central nervous system, in which traditional treatments are largely ineffective due to the complex nature of the injured tissue. Therefore, biomaterial-based systems have been developed as possible alternative strategies to repair the damaged tissue. In the present study, we aimed to design bioactive agent loaded scaffolds composed of two layers with distinct physical properties to improve tissue regeneration. An electrospun layer with aligned nanofibers was made of collagen (Col) Type-I, poly(lactide-co-glycolide) (PLGA) and laminin to promote cell attachment of mesenchymal-like stem cells towards the direction of fibers, while a Col-based second layer was fabricated by plastic compression to act as a releasing system for NT-3 and chondroitinase ABC, so that axonal growth could be stimulated. Results showed that a source of mesenchymal stem cell (MSC)-like cells, adipose tissue-derived stem cells cultured on the fibrous layer...

中文翻译：

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一种使用多功能胶原基质修复脊髓损伤的组合方法：开发、表征和对细胞粘附和轴突生长的影响。

脊髓损伤是中枢神经系统的一种破坏性疾病，由于受伤组织的复杂性，传统治疗在很大程度上是无效的。因此，已经开发了基于生物材料的系统作为修复受损组织的可能替代策略。在本研究中，我们旨在设计由具有不同物理特性的两层组成的生物活性剂负载支架，以改善组织再生。具有对齐纳米纤维的电纺层由 I 型胶原 (Col)、聚(丙交酯-共-乙交酯) (PLGA) 和层粘连蛋白制成，以促进间充质样干细胞向纤维方向的细胞附着，而 Col-基于的第二层是通过塑性压缩制造的，作为 NT-3 和软骨素酶 ABC 的释放系统，从而可以刺激轴突生长。