We performed RNA sequencing on Bordetella pertussis , the causative agent of whooping cough, and identified nine novel small RNAs (sRNAs) that were transcribed during the bacterial colonization of murine tracheas. Among them, four sRNAs were more strongly expressed in vivo than in vitro . Moreover, the expression of eight sRNAs was not regulated by the BvgAS two‐component system, which is the master regulator for the expression of genes contributing to the bacterial infection. The present results suggest a BvgAS‐independent gene regulatory system involving the sRNAs that is active during B. pertussis infection.

中文翻译：

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百日咳博德特氏菌小RNA定居鼠气管小RNA的表达。

我们对百日咳百日咳百日咳博德特氏菌进行了RNA测序，并鉴定了9种在鼠气管细菌定植过程中转录的新颖小RNA（sRNA）。其中，四种sRNA在体内比在体外表达更强。此外，八种sRNA的表达不受BvgAS两组分系统的调节，BvgAS两组分系统是导致细菌感染的基因表达的主要调节剂。目前的结果表明，涉及百日咳博德特氏菌感染期间活跃的sRNA的BvgAS独立基因调控系统。