The G protein-coupled receptor is one of the major transmembrane proteins in plants. It consists of an α subunit, a β subunit and three γ subunits. Chilling tolerant divergence 1 (COLD1) includes a Golgi pH receptor (GPHR) domain, which maintains cell membrane organisation and dynamics, along with abscisic acid linked G protein-coupled receptor (ABA_GPCR) that regulates the signalling pathways during cold stress. In the present study, we performed characterisation of a homologous COLD1 from the economically important monocot species Oryza sativa L., Zea mays L., Sorghum bicolor (L.)Moench and Erianthus arundinaceus (L.) Beauv. IK 76-81, a wild relative of Saccharum. COLD1 was isolated from E. arundinaceus IK 76-81, analysed for its evolution, domain, membrane topology, followed by prediction of secondary, tertiary structures and functionally validated in all four different monocots. Gene expression studies of COLD1 revealed differential expression under heat, drought, salinity and cold stresses in selected monocots. This is the first study on regulation of native COLD1 during abiotic stress in monocots, which has opened up new leads for trait improvement strategies in this economically important crop species.

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更正内容：非生物胁迫中单子叶植物的耐冷差异1（COLD1）基因的计算机表征和功能验证。

G蛋白偶联受体是植物中主要的跨膜蛋白之一。它由一个α亚基，一个β亚基和三个γ亚基组成。耐冷差异1（COLD1）包括一个高尔基pH受体（GPHR）结构域，该结构域维持细胞膜的组织和动态，以及脱落酸连接的G蛋白偶联受体（ABA_GPCR），该受体在寒冷胁迫下调节信号传导途径。在本研究中，我们对经济上重要的单子叶植物Oryza sativa L.，Zea mays L.，高粱双色（L.）Moench和Erianthus arundinaceus（L.）Beauv的同源COLD1进行了表征。IK 76-81，蔗糖的野生近缘种。COLD1是从分析了大肠埃希菌IK 76-81的进化，结构域，膜拓扑，随后预测了二级，三级结构，并在所有四个不同的单子叶植物中进行了功能验证。COLD1的基因表达研究表明，在选定的单子叶植物中，在高温，干旱，盐度和寒冷胁迫下差异表达。这是关于单子叶植物非生物胁迫期间天然COLD1调控的第一项研究，为该经济上重要的作物物种的性状改良策略开辟了新的线索。