Bone cancer pain (BCP) is a common chronic pain that is caused by a primary or metastatic bone tumor. More detailed molecular mechanisms of BCP are warranted. In this study, we established a BCP rat model. The von Frey hair test, body weight, and hematoxylin and eosin staining were employed. We screened differentially expressed circRNAs (DECs) between the BCP group and sham group. The results revealed that 850 DECs were significantly up-regulated and 644 DECs were significantly down-regulated in the BCP group. Furthermore, we identified 1177 differentially expressed genes (DEGs) significantly up-regulated and 565 DEGs significantly down-regulated in the BCP group. Gene Ontology annotation of all 1742 DEGs revealed that biological regulation of metabolic processes, cellular processes, and binding were the top enriched terms. For Kyoto Encyclopedia of Genes and Genomes analysis, phagosome, HTLV-I infection, proteoglycans in cancer, and herpes simplex infection were significantly enriched in this study. In addition, we identified four selected circRNAs, chr6:72418120|72430205, chr20:7561057|7573740, chr18:69943105|69944476, and chr5:167516581|167558250, by quantitative real time PCR. chr6:72418120|72430205 (circStrn3) was selected for further study based on expression level and the circRNA–miRNA–mRNA network table. Western blot analysis suggested that knockdown of circStrn3 could effectively induce Walker 256 cell apoptosis. In summary, our study provided a more in-depth understanding of the molecular mechanisms of BCP.

中文翻译：

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circStrn3与大鼠模型中的骨癌疼痛调节有关。

骨癌疼痛（BCP）是由原发性或转移性骨肿瘤引起的常见慢性疼痛。BCP的分子机制更加详细。在这项研究中，我们建立了BCP大鼠模型。使用冯·弗雷头发测试，体重以及苏木精和曙红染色。我们筛选了BCP组和假组之间的差异表达circRNA（DECs）。结果显示，BCP组的850 DEC明显上调，而644 DEC明显下调。此外，我们在BCP组中发现了1177个差异表达基因（DEG）显着上调，而565个DEGs显着下调。所有1742个DEG的基因本体论注释显示，代谢过程，细胞过程和结合的生物调节是最富裕的术语。对于《京都市基因与基因组百科全书》的分析，吞噬体，HTLV-1感染，癌症中的蛋白聚糖和单纯疱疹感染在本研究中得到了显着丰富。此外，我们通过实时定量PCR鉴定了四个选定的circRNA，即chr6：72418120 | 72430205，chr20：7561057 | 7573740，chr18：69943105 | 69944476和chr5：167516581 | 167558250。根据表达水平和circRNA–miRNA–mRNA网络表，选择了chr6：72418120 | 72430205（circStrn3）进行进一步研究。蛋白质印迹分析表明，敲除circStrn3可以有效诱导Walker 256细胞凋亡。总之，我们的研究对BCP的分子机制提供了更深入的了解。和单纯疱疹感染大大丰富了这项研究。此外，我们通过实时定量PCR鉴定了四个选择的circRNA，即chr6：72418120 | 72430205，chr20：7561057 | 7573740，chr18：69943105 | 69944476和chr5：167516581 | 167558250。根据表达水平和circRNA–miRNA–mRNA网络表，选择了chr6：72418120 | 72430205（circStrn3）进行进一步研究。蛋白质印迹分析表明，敲除circStrn3可以有效诱导Walker 256细胞凋亡。总之，我们的研究对BCP的分子机制提供了更深入的了解。和单纯疱疹感染大大丰富了这项研究。此外，我们通过实时定量PCR鉴定了四个选定的circRNA，即chr6：72418120 | 72430205，chr20：7561057 | 7573740，chr18：69943105 | 69944476和chr5：167516581 | 167558250。根据表达水平和circRNA–miRNA–mRNA网络表，选择了chr6：72418120 | 72430205（circStrn3）进行进一步研究。蛋白质印迹分析表明，敲除circStrn3可以有效诱导Walker 256细胞凋亡。总之，我们的研究对BCP的分子机制提供了更深入的了解。根据表达水平和circRNA–miRNA–mRNA网络表，选择72418120 | 72430205（circStrn3）进行进一步研究。蛋白质印迹分析表明，敲除circStrn3可以有效诱导Walker 256细胞凋亡。总之，我们的研究对BCP的分子机制提供了更深入的了解。根据表达水平和circRNA–miRNA–mRNA网络表，选择72418120 | 72430205（circStrn3）进行进一步研究。蛋白质印迹分析表明，敲除circStrn3可以有效诱导Walker 256细胞凋亡。总之，我们的研究对BCP的分子机制提供了更深入的了解。