AIM AND PURPOSE The higher prevalence rate of different diseases may accentuate the possible alteration of the immune system in individuals conceived through the assisted reproductive technologies (ART). The present study was conducted to evaluate the TH1, TH2, TH17 balance in intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) - conceived mice in comparison to naturally conceived offspring. METHODS Mice (6-8 weeks) were divided into three groups (IVF- conceived, ICSI- conceived and naturally conceived). They were subjected to subcutaneous immunization witMycobacterium bovis Bacille Calmette-Guérin (BCG). The blood samples were taken and the sera were separated. Then the spleens were surgically removed at the time the mice were sacrificed. Serum levels of IFN-γ, IL-17A and IL-4 were detected by ELISA. Then the proportion of TCD4 cells possessing the T-bet+, GATA3+, and ROR-γt + were measured using FACS caliber flow cytometer. RESULTS In comparison with naturally conceived mice, intracellular expression of T-bet and serum levels of IFN-γ were significantly decreased in ART- conceived mice. Moreover, ART- conceived offspring showed marked increase in IL-4 and IL-17A. CONCLUSION It is concluded that compared to naturally conceived male mice, ART- conceived counterparts exhibit less efficient immune responses against BCG through further promotion of humoral and inflammatory related immune response characteristics.

中文翻译：

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改变辅助生殖技术受孕小鼠中的 TH1、TH2、TH17 平衡。

目的和目的 不同疾病的较高患病率可能会加剧通过辅助生殖技术 (ART) 受孕的个体的免疫系统可能发生的变化。本研究旨在评估卵胞浆内单精子注射 (ICSI) 和体外受精 (IVF) 受孕小鼠与自然受孕后代的 TH1、TH2、TH17 平衡。方法 将小鼠（6-8 周）分为三组（IVF 受孕、ICSI 受孕和自然受孕）。他们用牛分枝杆菌卡介苗（BCG）进行皮下免疫。采集血样并分离血清。然后在处死小鼠时通过手术切除脾脏。通过ELISA检测血清IFN-γ、IL-17A和IL-4水平。然后使用FACS口径流式细胞仪测量具有T-bet+、GATA3+和ROR-γt+的TCD4细胞的比例。结果 与自然受孕小鼠相比，ART 受孕小鼠的 T-bet 细胞内表达和 IFN-γ 血清水平显着降低。此外，ART 受孕的后代显示出 IL-4 和 IL-17A 的显着增加。结论 结论是，与自然受孕的雄性小鼠相比，ART 受孕的对应物通过进一步促进体液和炎症相关的免疫反应特征表现出针对 BCG 的低效免疫反应。在 ART 受孕小鼠中，T-bet 的细胞内表达和 IFN-γ 的血清水平显着降低。此外，ART 受孕的后代显示出 IL-4 和 IL-17A 的显着增加。结论 结论是，与自然受孕的雄性小鼠相比，ART 受孕的对应物通过进一步促进体液和炎症相关的免疫反应特征表现出针对 BCG 的低效免疫反应。在 ART 受孕小鼠中，T-bet 的细胞内表达和 IFN-γ 的血清水平显着降低。此外，ART 受孕的后代显示出 IL-4 和 IL-17A 的显着增加。结论 结论是，与自然受孕的雄性小鼠相比，ART 受孕的对应物通过进一步促进体液和炎症相关的免疫反应特征表现出针对 BCG 的低效免疫反应。