Urushiols are amphipathic compounds found in Rhus verniciflua Stokes that exhibit various biological activities. However, their practical use is very restricted due to their contact dermatitis-inducing property. Therefore, we applied the ionization method to remove the allergenic properties of the urushiols and to increase their usability. One of the natural urushiols, 3-pentadecylcatechol (PDC), was heated for 30 min with a solution of H₂O and sodium carbonate (Na₂CO₃). The reaction product was analyzed by electrospray ionization mass spectrometry (ESI-MS). Ionized PDC with an m/z value of 316.9 and complexed PDCs with Na⁺ of 1 − 3 atoms with m/z values of 340.8, 365.2, and 380.8 were detected. PDC and ionized PDC (3 μmol/3 mg of Vaseline) treatments were applied on the rear of left ear of Sprague-Dawley rats once daily for 10 days. Erythema and swelling were observed on the ear skin treated with PDC, but not in case of ionized PDC. Compared with control, contact hypersensitivity-related biomarkers (neutrophils, eosinophils, immunoglobulin E, and histamine) in the blood were significantly higher only in the PDC-treated group. In addition, Il-1b, Il-6, Tnfα, and Cox-2 mRNA expression levels were dramatically increased in the ear tissue of PDC-treated rats, but in the ionized PDC-treated group, they were similar to those in the control group. Overall, it was confirmed that the allergenic property of the urushiol PDC was removed by ionization. This method is expected to be useful for preventing allergy induction in cooking and food processing using R. verniciflua Stokes.

中文翻译：

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电离可中和3-戊基邻苯二酚的过敏诱导特性：一种漆酚衍生物。

漆酚是在Rus verniciflua Stokes中发现的两亲化合物，具有多种生物活性。然而，由于它们引起接触性皮炎的性质，它们的实际使用受到很大限制。因此，我们应用了离子化方法去除了漆酚的变应原性并增加了其可用性。用H ₂ O和碳酸钠（Na ₂ CO ₃）的溶液将天然的漆酚中的一种，3-戊基邻苯二酚（PDC）加热30分钟。通过电喷雾电离质谱法（ESI-MS）分析反应产物。离子化的PDC具有M / Z的值316.9和络合的PDC用Na ⁺ 1 - 3原子与M / Z检测到340.8、365.2和380.8的值。每天一次对Sprague-Dawley大鼠的左耳后部进行PDC和离子化PDC（  3μmol / 3 mg凡士林）处理，持续10天。在用PDC处理的耳朵皮肤上观察到红斑和肿胀，但在电离PDC情况下则没有。与对照组相比，仅PDC治疗组的血液中接触超敏反应相关的生物标志物（嗜中性粒细胞，嗜酸性粒细胞，免疫球蛋白E和组胺）明显更高。此外，Il-1b，Il-6，Tnfα和Cox-2在PDC处理的大鼠的耳朵组织中，mRNA的表达水平显着增加，但是在电离的PDC处理的组中，它们与对照组相似。总的来说，证实了通过电离除去了漆酚PDC的变应原性。预期该方法可用于预防在使用黑斑病Stokes的烹饪和食品加工中引起过敏。