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Quantitative Proteomic Analysis of MARC-145 Cells Infected with a Mexican Porcine Reproductive and Respiratory Syndrome Virus Strain Using a Label-Free Based DIA approach.
Journal of the American Society for Mass Spectrometry ( IF 3.2 ) Pub Date : 2020-05-21 , DOI: 10.1021/jasms.0c00134 Emmanuel Ríos-Castro 1 , Gustavo Henrique Martins Ferreira Souza 2 , Dulce María Delgadillo-Álvarez 1 , Lorena Ramírez-Reyes 1 , Ana Laura Torres-Huerta 3 , Andrea Velasco-Suárez 1 , Carlos Cruz-Cruz 4 , José Manuel Hernández-Hernández 5 , José Tapia-Ramírez 4
Journal of the American Society for Mass Spectrometry ( IF 3.2 ) Pub Date : 2020-05-21 , DOI: 10.1021/jasms.0c00134 Emmanuel Ríos-Castro 1 , Gustavo Henrique Martins Ferreira Souza 2 , Dulce María Delgadillo-Álvarez 1 , Lorena Ramírez-Reyes 1 , Ana Laura Torres-Huerta 3 , Andrea Velasco-Suárez 1 , Carlos Cruz-Cruz 4 , José Manuel Hernández-Hernández 5 , José Tapia-Ramírez 4
Affiliation
Porcine reproductive and respiratory syndrome (PRRS) is an infectious disease characterized by severe reproductive failure in sows, acute respiratory disorders in growing pigs, and high mortality in piglets. The causative agent of this syndrome is the PRRS virus (PRRSV), an RNA virus belonging to the Arteriviridae family. To date, several quantitative approaches of proteomics have been applied to analyze the gene expression profiles during PRRSV infection in PAMs and MARC-145 cells, and few proteins have been consistent among independent studies, probably due to the differences in the levels of virulence of different PRRSV strains used and/or due to analytical conditions. In this study, total proteins isolated from noninfected and infected MARC-145 cells with a Mexican PRRSV strain were relatively quantified using label-free based DIA approach in combination with ion-mobility separation. As a result, 1456 quantified proteins were found to be shared between the control and infected samples. Afterward, these proteins were filtered, and 699 of them were considered without change. Also, 17 proteins were up-regulated and 19 proteins were down-regulated during the PRSSV infection. Bioinformatic analysis revealed that many of the differentially expressed proteins are involved in processes like antigen processing, presentation of antigens, response to viruses, response to IFNs, and innate immune response, among others. The present work is the first one which provides a detailed proteomic analysis through label-free based DIA approach in MARC-145 cells during the infection with a Mexican PRRSV strain.
中文翻译:
使用基于无标记的DIA方法的墨西哥猪繁殖与呼吸综合征病毒株感染的MARC-145细胞的蛋白质组学定量分析。
猪繁殖与呼吸综合症(PRRS)是一种传染病,其特征是母猪严重繁殖衰竭,生长中的猪出现急性呼吸系统疾病以及仔猪的高死亡率。该综合征的病原体是PRRS病毒(PRRSV),它是Arteriviridae家族的RNA病毒。迄今为止,蛋白质组学的几种定量方法已用于分析PAM和MARC-145细胞在PRRSV感染过程中的基因表达谱,并且独立研究之间几乎没有蛋白质一致,这可能是由于不同毒力水平的差异所致。使用的PRRSV菌株和/或由于分析条件。在这个研究中,使用基于无标记的DIA方法结合离子迁移分离技术,相对定量地分析了未感染和感染墨西哥PRRSV病毒的MARC-145细胞的总蛋白。结果,发现对照样品和感染样品之间共有1456个定量蛋白质。之后,将这些蛋白质过滤,其中699个被认为没有变化。另外,在PRSSV感染期间,有17种蛋白质被上调,而19种蛋白质被下调。生物信息学分析表明,许多差异表达的蛋白质都参与了诸如抗原加工,抗原呈递,对病毒的反应,对IFN的反应以及先天免疫反应等过程。
更新日期:2020-05-07
中文翻译:
使用基于无标记的DIA方法的墨西哥猪繁殖与呼吸综合征病毒株感染的MARC-145细胞的蛋白质组学定量分析。
猪繁殖与呼吸综合症(PRRS)是一种传染病,其特征是母猪严重繁殖衰竭,生长中的猪出现急性呼吸系统疾病以及仔猪的高死亡率。该综合征的病原体是PRRS病毒(PRRSV),它是Arteriviridae家族的RNA病毒。迄今为止,蛋白质组学的几种定量方法已用于分析PAM和MARC-145细胞在PRRSV感染过程中的基因表达谱,并且独立研究之间几乎没有蛋白质一致,这可能是由于不同毒力水平的差异所致。使用的PRRSV菌株和/或由于分析条件。在这个研究中,使用基于无标记的DIA方法结合离子迁移分离技术,相对定量地分析了未感染和感染墨西哥PRRSV病毒的MARC-145细胞的总蛋白。结果,发现对照样品和感染样品之间共有1456个定量蛋白质。之后,将这些蛋白质过滤,其中699个被认为没有变化。另外,在PRSSV感染期间,有17种蛋白质被上调,而19种蛋白质被下调。生物信息学分析表明,许多差异表达的蛋白质都参与了诸如抗原加工,抗原呈递,对病毒的反应,对IFN的反应以及先天免疫反应等过程。