Chemical probes are essential tools used to study and modulate biological systems. Here, we describe some of the recent scientific advancement in the field of chemical biology, as well as how the advent of new technologies is redefining the criteria of 'good' chemical probes and influencing the discovery of valuable drug leads. In this review, we report selected examples of the usage of linkered and linker-free chemical probes for target identification, biological discovery, and general mechanistic understanding. We also discuss the promises of chemogenomics libraries in phenotypic screens, as well as the limitation of their usage to identify the modulation of new targets and biology.

中文翻译：

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“设计，合成和战略性地使用小型化学探针来鉴定药物开发的新靶标”。

化学探针是用于研究和调节生物系统的重要工具。在这里，我们描述了化学生物学领域的一些最新科学进展，以及新技术的出现如何重新定义“优质”化学探针的标准并影响了有价值的药物线索的发现。在这篇综述中，我们报告了一些使用接头和无接头化学探针进行靶标鉴定，生物学发现和一般机理理解的实例。我们还讨论了在表型筛选中化学基因组学文库的前景，以及它们在识别新靶标和生物学调控方面的使用限制。