Spent mushroom substrate (SMS) of Pleurotus ostreatus was supplemented with wheat bran and soybean flour and used as substrate for a new cultivation cycle of the oyster mushrooms Pleurotus ostreatus and Pleurotus pulmonarius. The bioconversion efficiency of mushrooms produced over substrate (BE%) used and the chemical composition of sporophores were evaluated. The concentration of mycelial mass, crude exopolysaccharide content and laccase enzyme activity were also determined at the supplemented SMS before inoculation, at 50% and 100% of colonization stages in the new cultivation and in the final re-utilized SMS. The laccase enzyme was extracted to examine SMS potential for the dephenolization of olive mill and winery wastewaters. Results showed that both Pleurotus species exhibited BE over 185%, demonstrating this bioprocess could represent a promising strategy to convert SMS into nutritional food. Data also indicate the strong positive impact that SMS could have in the solid wastes' management and agribusiness enhancement.

中文翻译：

---

用过的蘑菇底物用于平菇的第二个栽培周期和农业工业废水的脱酚。

平菇平菇的废蘑菇底物（SMS）补充有麦麸和大豆粉，并用作平菇菇和平菇的新栽培周期的底物。评价了所使用的底物上所产生的蘑菇的生物转化效率（BE％）和孢子体的化学组成。还测定了接种前补充的SMS，新栽培和最终再利用的SMS中定植阶段的50％和100％的菌丝质量浓度，粗制胞外多糖含量和漆酶活性。提取了漆酶，以检查SMS潜力对橄榄油厂和酿酒厂废水的脱酚作用。结果表明，两种杏鲍菇均具有超过185％的BE，证明这种生物过程可能是将SMS转化为营养食品的有前途的策略。数据还表明，SMS可能会对固体废物的管理和农业综合企业的发展产生积极的积极影响。