Green-emitting water-soluble amino-ketoenole dye AmyGreen is proposed as an efficient fluorescent stain for visualization of bacterial amyloids in biofilms and the detection of pathological amyloids in vitro . This dye is almost non-fluorescent in solution, displays strong green emission in the presence of amyloid fibril of proteins. AmyGreen is also weakly fluorescent in presence to biomolecules that are components of cells, extracellular matrix or medium: nucleic acids, polysaccharides, lipids, and proteins. Thus, the luminescence turn-on behavior of AmyGreen can be utilized for visualization of amyloid components of bacterial biofilm extracellular matrix. Herein we report the application of AmyGreen for fluorescent staining of a number of amyloid-contained bacteria biofilms produced by Escherichia coli , Klebsiella pneumoniae, Pseudomonas aeruginosa , Bordetella avium , and Staphylococcus aureus . The effectiveness of AmyGreen was compared to tra...

中文翻译：

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荧光β-酮烯醇AmyGreen染料，用于可视化细菌生物膜的淀粉样成分。

提出了绿色发光的水溶性氨基酮烯染料AmyGreen作为一种有效的荧光染料，用于可视化生物膜中的细菌淀粉样蛋白并在体外检测病理性淀粉样蛋白。这种染料在溶液中几乎是无荧光的，在蛋白质的淀粉样蛋白原纤维存在下显示出强绿色发射。AmyGreen在存在作为细胞，细胞外基质或培养基（核酸，多糖，脂质和蛋白质）组成部分的生物分子时，也会发出微弱的荧光。因此，AmyGreen的发光开启行为可用于可视化细菌生物膜细胞外基质的淀粉样成分。在此，我们报告了AmyGreen在荧光染色中的应用，这些荧光染色由大肠杆菌，肺炎克雷伯菌，铜绿假单胞菌，博德特氏菌和金黄色葡萄球菌。将AmyGreen的功效与贸易...