Ginseng (G) and Prepared Rehmannia Root (PRR) are commonly used in traditional Chinese medicine for blood supplementation. This study aimed to study G and PRR with different compatibility ratios changes in chemical composition and inhibition of cyclophosphamide-induced myelosuppression. HPLC was used to determine the chemical constituents of 13 ginsenosides, 5-hydroxymethylfurfural (5-HMF) and verbascoside in different proportions of G-PRR. Balb/c mice were injected intraperitoneally with cyclophosphamide (CTX) to induce bone marrow suppression. The effects of different proportions of G-PRR on peripheral blood, bone marrow nucleated cells, thymus and spleen index of myelosuppressed mice were analyzed. The results showed that the compatibility of G and PRR can promote the dissolution of ginsenosides, and the content of conventional ginsenosides decreased, and the content of rare ginsenosides increased. Different proportions of G-PRR increased the number of peripheral blood and bone marrow nucleated cells in cyclophosphamide-induced bone marrow suppression mice (p < 0.01), increased thymus index (p < 0.01), decreased spleen index (p < 0.01). Different proportions of G-PRR can improve the myelosuppression induced by cyclophosphamide in mice, and the combined effect of G-PRR is better than the single decoction of G and PRR. Among them, G-PRR 2 : 3 and G-PRR 1 : 2 were better than the other groups. These results indicate that different proportion of G-PRR can improve bone marrow suppression, and the combined decoction of G-PRR is better than the separate Decoction in improving bone marrow suppression. This improvement may be related to the changes of the substance basis and active ingredients of G-PRR.

中文翻译：

---

人参制地黄不同配伍中化学成分的变化及其对化疗后骨髓抑制作用的影响。

人参（G）和熟地黄（PRR）通常在传统中药中用于补血。这项研究旨在研究具有不同配比的G和PRR的化学成分变化和对环磷酰胺诱导的骨髓抑制的抑制作用。HPLC用于测定不同比例的G-PRR中13种人参皂苷，5-羟甲基糠醛（5-HMF）和马齿bas苷的化学成分。向Balb / c小鼠腹膜内注射环磷酰胺（CTX）以诱导骨髓抑制。分析了不同比例的G-PRR对骨髓抑制小鼠外周血，骨髓有核细胞，胸腺和脾脏指数的影响。结果表明，G和PRR的相容性可以促进人参皂苷的溶解，常规人参皂苷含量降低，稀有人参皂苷含量增加。不同比例的G-PRR增加了环磷酰胺诱导的骨髓抑制小鼠的外周血和骨髓有核细胞的数量（p <0.01），胸腺指数增加（p <0.01），脾脏指数降低（p <0.01）。不同比例的G-PRR可以改善环磷酰胺诱导的小鼠骨髓抑制，G-PRR的联合作用优于G和PRR的单次煎剂。其中，G-PRR 2：3和G-PRR 1：2优于其他组。这些结果表明，不同比例的G-PRR可以改善骨髓抑制，并且G-PRR的联合汤优于单独的汤在改善骨髓抑制方面。